摘要
目的探讨高原环境对睡眠剥夺(SD)大鼠学习记忆及海马5-HT表达的影响。方法64只大鼠被随机分为可可西里组(海拔4767m)和兰州组(海拔1520m)。各组大鼠又分为正常睡眠及SD1d、3d和5d4个亚组。采用小平台水环境法(flowerpot)建立大鼠SD模型。各组大鼠行Morris水迷宫测试,免疫组化法检测海马组织中5-HT的含量。结果Morris水迷宫显示,在可可西里组和兰州组中,与正常睡眠大鼠比较SD1d、3d及5d大鼠的潜伏期延长(P<0.05),游泳路程增加(P<0.05);以及穿越平台次数减少(P<0.05)。与兰州组比较,可可西里组的正常睡眠及SD1d、3d和5d大鼠的潜伏期延长(P<0.05),游泳路程增加(P<0.05),穿越平台次数减少(P<0.05或P<0.01)。海马5-HT含量检测中可可西里组SD1d、3d及5d大鼠的含量均高于正常睡眠大鼠(P<0.05);兰州组SD3d及5d大鼠的含量均高于正常睡眠大鼠(P<0.05);与兰州组相比,可可西里组正常睡眠、SD1d、3d和5d大鼠的5-HT含量增加(P<0.05)。结论高原环境暴露可使SD大鼠学习记忆能力进一步降低,其机制可能与海马中5-HT含量增高有关。
Objective To instigative the effects of high-altitude environment on cognitive function and 5-hydroxytryptamine（5-HT）content in the hippocampus in rats with sleep deprivation.Methods Sixty-four rats were randomly divided into Kekexili（altitude：4767 m）and Lanzhou（altitude：1520 m）groups.They received normal sleep or were subjected to sleep deprivation for 1,3 or 5 days.Sleep deprivation was induced by ＂flower pot＂ technique.The behaviors of rats were studied by Morris maze test.Immunohistochemistry was used to detect the content of 5-HT in the hippocampus.Results The Morris water maze test showed that the escape latency prolonged,swimming distance increased and the times of crossing the exact former platform location decreased both in the Kekexili and Lanzhou groups after 1,3 or 5 days of sleep deprivation compared with those in rats with normal sleep（P0.05）.The Kekexili group had prolonged escape latency,increased swimming distance and decreased times of crossing the exact former platform location compared with the Lanzhou group under normal sleep and 1,3 or 5 days of sleep deprivation（P0.05）.The 5-HT contents were higher in the Kekexili group 1,3 or 5 days after sleep deprivation than those in rats with normal sleep（P0.05）.In the Lanzhou group,the 5-HT contents 3 or 5 days after sleep deprivation increased compared with those in rats with normal sleep（P0.05）.The Kekexili group had increased 5-HT contents compared with the Lanzhou group under normal sleep and 1,3 or 5 days of sleep deprivation（P0.05）.Conclusions High-altitude environment exposure may decrease cognitive function in rats with sleep deprivation.An increase in 5-HT contents in the hippocampus might be one of the underlying mechanisms.
出处
《国际神经病学神经外科学杂志》
2010年第2期107-110,共4页
Journal of International Neurology and Neurosurgery
基金
全军"十一五"科技攻关项目(08G010)
