摘要
目的:观察Kiss-1蛋白及mRNA在食管鳞状细胞癌组织中的表达及其临床病理意义.方法:采用免疫组织化学SP法、原位杂交和逆转录聚合酶链反应(RT-PCR)技术,联合检测了62例食管鳞状细胞癌、31例癌旁不典型增生及62例正常食管组织中Kiss-1蛋白和mRNA的表达,并探讨其临床病理意义.结果:在食管鳞状细胞癌组织、癌旁不典型增生及正常食管组织中,Kiss-1蛋白的阳性表达率分别为56.5%、67.7%、90.3%(P<0.05);用原位杂交检测Kiss-1mRNA阳性表达率分别为51.6%、74.2%、95.2%(P<0.05);用RT-PCR技术检测Kiss-1mRNA阳性表达率分别为54.8%、71.0%、88.7%(P<0.05);食管鳞状细胞癌组织中Kiss-1蛋白及mRNA的低表达与淋巴结转移密切相关(P<0.05),而与患者的性别、年龄、肿瘤的组织学分级无关(P>0.05);Kiss-1蛋白的低表达与浸润深度有关(P<0.05);在食管鳞状细胞癌组织中,Kiss-1蛋白及mRNA的表达呈正相关(P<0.05),用原位杂交及RT-PCR技术对Kiss-1mRNA表达情况的检测结果也呈正相关(P<0.05).结论:Kiss-1基因的表达降低或缺失和食管鳞状细胞癌的发生发展及转移有关,有望成为食管鳞状细胞癌早期诊断和预后判断的重要指标之一.
AIM：To investigate the expression of Kiss-1 mRNA and protein in esophageal squamous cell carcinoma （ESCC） and to analyze its clinicopathological significance.METHODS：Sixty-two ESCC specimens,31 atypical hyperplastic epithelial specimens,and 62 normal esophageal epithelial specimens were used in the study.The expression of Kiss-1 mRNA in these specimens was detected by in situ hybridization and reverse transcription-polymerase chain reaction （RT-PCR）.The expression of Kiss-1 was detected by immunohistochemistry using the streptavidin-peroxidase method.The clinicopathological significance of Kiss-1 expression in ESCC was then analyzed.RESULTS：The positive rates of Kiss-1 protein expression in ESCC,adjacent atypical hyperplastic epithelium and normal esophageal epithelium tissues were 56.5%,67.7%,90.3%,respectively （all P〈0.05）.The positive rates of Kiss-1 mRNA expression in ESCC,adjacent atypical hyperplastic epithelium and normal esophageal epithelium tissues were 51.6%,74.2% and 95.2%,respectively,as revealed by in situ hybridization （all P〈0.05）,and 54.8%,71.0% and 88.7%,respectively,by RT-PCR （all P〈0.05）.The positive rates of Kiss-1 protein and mRNA expression in ESCC were closely associated with lymph node metastasis （both P〈0.05）,but not with sex,age,histological grade and infiltration depth （all P〈0.05）.A positive correlation was noted not only between the positive rates revealed by in situ hybridization and RT-PCR （P〈0.05）,but also between those revealed by immunohistochemistry and RT-PCR or in situ hybridization （both P〈0.05）.CONCLUSION：The low expression of Kiss-1 is closely associated with the development,progression and metastasis of ESCC.Kiss-1 may be used as an important parameter for early diagnosis and prognosis of ESCC.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第10期1004-1009,共6页
World Chinese Journal of Digestology
基金
河南省高校杰出科研人才创新工程基金资助项目
No.2007KYCX005~~
