摘要
目的:探讨粒细胞集落刺激因子(granulocytecolony-stimulatingfactor,G-CSF)促进肝大部切除小鼠肝再生的作用.方法:建立小鼠肝大部分切除(约70%)模型,将肝切除小鼠随机分为3组.单纯肝切除组:肝切除后24h,腹腔注射生理盐水5d;G-CSF+肝切除组:rhG-CSF150μg/(kg?d)腹腔注射5d,24h后进行肝切除;肝切除+G-CSF组:肝切除术后24h,rhG-CSF150μg/(kg?d)腹腔注射5d.于术后7d取血清和肝组织,用全自动生化分析仪检测血清肝功能指标,并采用免疫组织化学方法观察肝内的增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)的表达及BrdU阳性细胞.结果:肝切除+G-CSF组和G-CSF+肝切除组BrdU及PCNA表达及BrdU阳性细胞明显升高,与对照组比较差异均具有统计学意义(PCNA:74.08%±8.86%,68.91%±9.64%vs57.36%±13.37%,均P<0.05);肝组织病理检查发现G-CSF+肝切除组27%(3/11)小鼠肝组织出现了不同程度的炎症反应,肝功生化学也显示血清ALT及AST升高,且其差异有统计学意义(均P<0.05).结论:G-CSF具有明显促进肝大部切除小鼠肝再生的作用,但也容易诱发肝脏炎症反应.
AIM： To investigate the effect of granulocyte colony-stimulating factor （G-CSF） on liver regeneration after major hepatectomy in mice. METHODS： Mice were subjected to 70% hepatectomy and randomly divided into three groups： control group （intraperitoneally injected with normal saline 24 h after hepatectomy, once a day for 5 d）, G-CSF pretreatment group [intraperitoneally injected with recombinant human G-CSF （rhG-CSF） at 150 μg/kg body weight daily for 5 d and underwent hepatectomy 24 h later], and G-CSF treatment group （intraperitoneally injected with rhG-CSF 24 h after hepatectomy, at 150 μg/kg body weight daily for 5 d）. Serum and hepatic tissue samples were harvested seven days after hepatectomy. Liver function parameters were measured using an automated biochemical analyzer. Immunohistochemistry was used to detect the expression of proliferating cell nuclear antigen （PCNA） and bromodeoxyuridine （BrdU）-positive cells. RESULTS： The percentages of PCNA-and BrdU-positive cells in the G-CSF pretreatment and treatment groups were significantly higher than those in the control group （PCNA： 74.08% ± 8.86% and 68.91% ± 9.64% vs 57.36% ± 13.37%, respectively; both P〈0.05）. Inflammatory response was noted in 27% （3/11） of mice in the G-CSF pretreatment group. Serum ALT and AST levels were significantly higher in the G-CSF pretreatment group than in the other two groups （both P〈0.05）. CONCLUSION： G-CSF promotes liver regeneration after major hepatectomy in mice, but G-CSF pretreatment induces infammatory response.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第11期1115-1120,共6页
World Chinese Journal of Digestology
基金
中国人民解放军南京军区医学科技创新基金资助项目
No.2007534~~
关键词
肝切除
粒细胞集落刺激因子
肝再生
炎症反应
Hepatectomy
Granulocyte colony-stimulating factor
Liver regeneration
Inflammatory response
