摘要
采用荧光分析技术和数学分析方法,对不同阶段鸡粪堆肥样品提取出的水溶性有机物进行了特征荧光参数定量化表征。结果显示:随着堆肥的进行,类腐殖质荧光峰与类蛋白荧光峰荧光强度的比值I330/I280、465nm激发波长下发射光谱中470～640nm范围内荧光积分面积A470～640及240nm激发波长下发射光谱中后四分之一波段与前四分之一波段的荧光强度积分面积比A435～480nm/A300～345nm均不断增大,表明堆肥腐殖化程度加大。三维荧光光谱显示,随着堆肥的进行类蛋白峰强度不断降低,而类富里酸峰强度不断增大,至堆肥结束类蛋白荧光基本消失;紫外区与可见区类富里酸峰荧光强度的比值r(A,C)随着堆肥的进行总体呈明显下降趋势,但出现了较大波动。相关性分析显示,I330/I280,A470～640及A435～480nm/A300～345nm两两间显著相关,而r(A,C)受其他因素影响较大,与上述3个参数未达到显著相关水平。结果表明,I330/I280,A470～640及A435～480nm/A300～345nm均能有效表征堆肥腐殖化进程。
Due to its high sensitivity,good selectivity and nondestructive nature,fluorescence spectroscopy was widely applied to characterize the nature of dissolved organic matter （DOM） from different source since the last decade.In the present study,dissolved organic matter （DOM） extracted from chicken manure samples during composting progress was quantitatively characterized by fluorescence analysis techniques and mathematical analysis methods.The results showed that,the ratio between the fluorescence intensities at 330 nm （humic-like matter） and 280 nm（protein-like matter） （I330/I280） in synchronous-scan excitation mode spectra,the area of a fluorescence spectrum obtained by excitation at the blue wavelength of 465 nm（A470-640）,and the ratio between the area of the last quarter （435-480 nm） and the area of the first quarter （300-345 nm） of the emission spectrum （A435-480 nm/A300-345 nm） by exciting the sample with ultraviolet radiation of 240 nm,all increased during composting,and the humification degree of compost increased as well.Three-dimensional excitation emission matrix fluorescence spectroscopy showed that the intensity of protein-like matter decreased during composting progress and diminished at the end,while that of fulvic-like matter increased all the time.The ratio between the intensity of the ultraviolet fulvic-like fluorescence （Peak A） and that of the visible fulvic-like fluorescence r（A,C） showed an overall significant downward trend during composting,but fluctuated in the progress.The correlation analysis showed that,I330/I280,A470-640 and A435-480 nm/A300-345 nm were all significant correlative,while r（A,C） was not correlative with the above-mentioned three parameters at 0.05 level due to effect by other factors,The results show that,I330/I280,A470-640 and A435-480 nm/A300-345 nm can be effectively used in the process of the evaluation of humification degree during composting.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2010年第6期1555-1560,共6页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(50878201)
水体污染控制与治理科技重大专项项目(2008ZX07209-007-004)
黑龙江省自然基金项目(C200901)
国家科技支撑计划课题项目(2007BAD87B03)资助
关键词
鸡粪
堆肥
水溶性有机物
定量化表征
Chicken manure
Composting
Dissolved organic matter
Quantitative characterization
