摘要
目的研究苦草Picriafel-terrae乙醇提取物30%乙醇洗脱部位(Pft-d)的抗癌活性。方法通过在体抗肿瘤实验和体外细胞毒实验,考察Pft-d对小鼠S180肉瘤、肝癌Heps肿瘤生长的抑制作用、对荷瘤小鼠免疫器官质量的影响及其细胞毒活性。结果 Pft-d对小鼠S180、Heps肿瘤的生长均有明显的抑制作用,剂量为10、20、40mg/(kg·d)时,对S180肉瘤生长的抑制率分别为44.44%、52.14%、61.97%,对肝癌Heps生长的抑制率分别为34.64%、41.55%、56.03%,与对照组比较差异显著(P<0.05、0.01、0.001)。对荷瘤小鼠免疫器官无明显影响。在体外,Pft-d高剂量时,对胃窦癌、胃癌、残骨癌等人癌细胞显示细胞毒活性,但低剂量时活性不明显。结论 Pft-d具有强的抗癌活性,值得进一步研究。
Objective To study the antitumor activity of Pft-d from Picria fel-terrae Lour. Methods Experiments of in vitro cell toxicity and in vivo antitumor were conducted. The inhibitive effect of Pft-d on tumor growth of sarcoma 180 （S180） and Heps were observed. The effects of Pft-d on immune organs of tumor-bearing mice were also studied. Results At the doses of 10, 20, and 40 mg/（kg · d）, the inhibitory rates of Pft-d on 8180 were 44.44%, 52. 14%, and 61.97% respectively, and the inhibitory rates on Heps were 34.64%, 41.55%, and 56.03% respectively. There were significant differences in the inhibitory rates between control group and experimental group （P〈0.05,P〈0.01 and P〈0.001）. However, there was no obvious effect on the immune organs of tumor-bearing mice. In vitro studies showed that high dose of Pft-d demonstrated cell toxic effects on patients of gastric antrum cancer, gastic cancer and so on. Low dose of Pft-d did not show obvious cell toxic effects. Conclusion Pft-d has significant antitumor activities on S180 and Heps, and worthy of further study.
出处
《现代药物与临床》
CAS
2010年第2期134-137,共4页
Drugs & Clinic
