摘要
目的研究下颌下腺肠三叶因子(intestinal trefoil peptide,ITF即TFF3)基因在大鼠实验性胃溃疡自愈过程的变化,探讨其与胃溃疡自愈的关系。方法通过胃窦前壁黏膜下注射冰乙酸制备大鼠胃溃疡模型:⑴用免疫组织化学SABC法和RT-PCR检测42只溃疡组,21只盐水组,及6只正常组大鼠下颌下腺组织中TFF3肽和TFF3mRNA的表达情况。结果(1)免疫组化显示:溃疡组大鼠下颌下腺的TFF3肽主要表达于导管系统上皮,如闰管、颗粒曲管(granular convoluted tubule,GCT)以及纹状管、小叶间导管上皮细胞、黏液腺泡细胞也有少量分布,浆液腺泡细胞呈阴性。溃疡组手术后第1d时,下颌下腺TFF3表达明显强于盐水组和正常组(P<0.01)。术后第2d,积分光密度明显低于1d溃疡组(P<0.05),4、6d积分光密度逐渐增强并高于对照组(P<0.05),到术后第10d达高峰(P<0.01),23d积分光密度仍高于对照组(P<0.05)。(2)RT-PCR显示:溃疡1、2、4、6、10、14、23dTFF3/GAPDH光密度比值分别为1.42±0.10,1.18±0.13,1.29±0.15,1.24±0.17,1.57±0.19,1.25±0.14,1.13±0.16明显高于相应盐水对照组的TFF3/GAP-DH光密度比值(P<0.01)。结论大鼠胃溃疡时期,下颌下腺TFF3基因上调。
Objective To explore the expression of trefoil factor3 （TFF3） gene in submaxilary glands during the self-healing of experimental gastric ulcer in rats. Methods Gastic ulcer was induced by injecting acetic acid to the mucosa of paries anterior gastricus. The expressions of TFF3 peptide and TFF3mRNA in 42 rats with gastric ulcer, 21 control rats and 6 normal rats were detected respectively by immunohistochemical SABC method and RT-PCR. Results （1）TFF3 immunoreactive cells were mainly located in the epithelium cytoplasm of intercalated ducts, GCT,striated ducts, and interlobular ducts,and in the mucous acinus,but not in serous acinus.TFF3 expression was stronger in gastric ulcer than in the other two groups on the first day（ P〈0.01）.The integral optical density （IOD） values decreased on the 2nd day when compared with that of the 1st day,then increased gradually on day 4 and 6,reached the peak on the 10th day（P〈0.01）, and kept a high level on the 23rd day compared with that of the corresponding control group （P〈0.05）. （2）The OD value of TFF3/GAPDH of the gastric ulcer group on 1,2,4,6,10,14,23d was 1.42±0.10,1.18±0.13,1.29±0.15,1.24±0.17, 1.57±0.19, 1.25±0.14,1.13±0.16,respectively.They were obviously higher than those of the saline control group（P〈0.01）.Conclusion The TFF3 gene is up-regulated in rat submaxilary glands,so we infer that TFF3 gene may participate in the regulation of self-healing in gastric ulcer.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2010年第1期26-31,共6页
Chinese Journal of Histochemistry and Cytochemistry
基金
河北省自然科学基金(C2007000906)
