摘要
目的通过研究血管内皮生长因子(vascular endometrial growth factor,VEGF)、缺氧诱导因子1α(hypoxia-inducible factor-1α,HIF-1α)和vasohibin在正常子宫内膜(normal endometrium,NE)和子宫内膜样腺癌(endom-etrioid adenocarcinoma,EA)组织中的表达,探讨上述指标与间质微血管密度(microvessel density,MVD)、EA临床病理特性及预后的关系。方法采用免疫组化Envision二步法检测105例EA和20例NE组织中VEGF、HIF-1α、vasohibin和MVD的表达。分析各指标相互之间及与EA临床病理特性和预后间的关系。结果(1)EA组中,VEGF、HIF-1α和MVD的表达明显高于NE组(P<0.05),vasohibin的表达明显低于NE组(P<0.01)。(2)VEGF、HIF-1α和MVD的表达与EA的手术病理分期相关(P<0.05)。(3)在EA组,除vasohibin外,VEGF、HIF-1α和MVD三者的表达量两两间均呈正相关(P<0.05)。(4)98例有完整随访资料患者的5年生存率为87.8%,VEGF、HIF-1α及vasohibin表达阳性组与阴性组5年生存率无显著性差异;MVD高表达组5年生存率明显低于MVD低表达组(P<0.05)。结论异常增生的微血管与EA发生、发展及预后有关。VEGF、HIF-1α、vasohibin三者间动态平衡的失调促进了EA组织中的微血管生成,重建三者间的正常调节关系,刺激vasohibin的表达,可能是EA治疗的一个靶点。
Objective To study the expression of vascular endometrial growth factor （VEGF）, hypoxia induced factor 1α（HIF-1α）,vasohibin and microvessel density（MVD） in normal endometriums （NE） and endometrioid adenocarcinoma （EA） tissues, And to explore their relationship to the biological and pathological feathers of EA. Methods Using immunohistochemiscal Envision method, VEGF, HIF-1α, vasohibin and MVD were detected in 105 EAs and 20 NEs. The relationship between these indications and the clinicopathological features were analyzed. Results The expressions of VEGF, HIF-1α and MVD were significantly higher in EA than in NE （P〈0.05）, while the expression of vasohibin was significantly lower in EA than in NE （P〈0.01）. Semi-quantitative analysis revealed that VEGF, HIF-1α and MVD expressions were significantly associated with the surgical pathologic staging （P〈0.05）. In the EA group, VEGF, HIF-1α and MVD had significant positive relationship between each other, while vasohibin had no significant relationship to the other three indications. The 5-year survival rate of the 98 cases of EC patients with intact followup materials was 87.8%. VEGF, HIF-1α and vasohibin were not related to the 5 year survival rate. The 5 year survival rate of paticnts with higher MVD was significantly lower than that of the group with lower MVD（P〈 0.05）.Conclusion The maladjustment among the expressions of VEGF, HIF-1α and vasohibin may promot angiogenesis in EA, and may be related to the surgical pathologic staging, which might be used to anticipate the prognosis of EA. Vasohibin could be a target of the treatment of EA.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2010年第1期43-48,共6页
Chinese Journal of Histochemistry and Cytochemistry
