摘要
目的研究纳洛酮对大鼠肺缺血-再灌注（ischemia／reperfusion injury，）损伤中细胞凋亡．I／R及血红素氯合酶－1（heine oxygenase－1，HO－1）表达的影响。方法实验于南京医科大学附属南京第一医院动物实验中心进行，雄性SD大鼠42只，随机（随机数字法）均分为对照组（Sh组）、缺血－再灌注组（1R组）、纳洛酮组（Na组）。I／R组钳夹肺门远心端，阻断肺门（以肺无舒缩为阻断标准），建立在体肺I／R损伤模型。Na组在该基础上予纳洛酮1mg·kg^-1腹腔注射，各组分别于3，6h点取标本，用Annexin—V-PI双染法检测肺组织凋亡细胞并计算凋亡率，测算肺湿干比（W／D），应用逆转录-聚合酶链反应（RT-PCR）法检测HO-1的mRNA表达，电镜观察肺超微结构改变。采用Stata9．0统计软件行统计学分析。结果IR组与Sh组相比，3，6h点肺组织凋亡率明显增加，W／D显著升高，差异均有统计学意义（P〈0．01）；IR组3，6h点，HO-1的mRNA表达与肺组织凋亡率、W／D呈显著负相关（P〈0．01）。与IR组柑比，Na组3，6h点肺组织凋亡率明显减少，W／D显著降低，差异均有统计学意义（P〈0．01），肺组织HO－1表达显著增加（P〈0．01），肺组织超微结构损害明显减轻。结论在肺I／R损伤早期，纳洛酬可以诱导HO-1的mRNA表达大量表达从而抑制细胞凋亡的发生，起到保护作用。
Objective To investigate the effects of naloxone （Na） on pneumocyte apoptosis and expression of heine oxygenas-1 （HO-1） during isehemia reperfusion injury of lung in rats. Method Fortv-twu male Sprague-Dawley rats were made models of ischemia reperfusion injury of unilateral lung, and were randondy（ randum number） divided into three groups： sham operation group （Sh group）, ischemia reperfusion group （IR group） and naloxone group （ Na gruup）. The hilus of lung was clamped for 45 minutes and the clamp was taken off to build the I/R model. After 3 - 6 hours reperfusion, naloxone in dose of 1 mg/kg was injected intra-peritoneally in rats of Na grnup. The rate of cell apoptosis in lung tissue was detected with the way of Annexin-V-Pl in flow cylometer. The wet to dry weight ratio （W/D） of lung tissue was measured. The expression of HO-1 in lung was measured by using RT-PCR and the ultra-structure change of lung tissue was observed under electron microscope. Results The rate of pneumocyte apoptosis and W/D ratio of lung tissue were significantly higher in IR group than in Sh group （ P 〈 0.01 ）, and the rate of pneumocyte apoptosis and W/D ratio of lung tissue were negatively correlated with the expression of HO-1 mRNA in lung tissue. Compared with IR group, the rate of cell apoptosis and W/D ratio were lower and the expression of HO-1 mRNA was higher in Na group （ P 〈 0.01 ）. The uhra-structure changes of lung tissue were lessened in Na group than in IR group. Conclusions During early period of lung IR injury, HO-1 induced by naloxone can inhibit the cellular apoptosis and protect the lung tissue.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2010年第5期507-510,共4页
Chinese Journal of Emergency Medicine
基金
基金项目：南京市医学科技发展重大项目（2007-3-6）
南京市科技发展计划项目（200601056）
南京市医学科技发展项目（YKK06063）
