摘要
目的探讨硫酸镍对体外培养小鼠精原细胞的氧化损伤作用。方法进行小鼠精原细胞的体外分离、纯化和培养,设置对照组和硫酸镍(NiSO4)50、100、200、400、800μmol/L5个测定组,等容积染毒后分别培养6、12、24和36h;在各染毒终点收割细胞,超声处理细胞悬液,离心取上清液,采用酶标仪检测总抗氧化能力(T-AOC)、羟自由基(-OH·)、谷胱甘肽过氧化物酶(GSH-Px)水平的变化。结果镍可引起精原细胞-OH·含量升高,T-AOC、GSH-Px活性降低。相同染毒时间,NiSO4各浓度组与对照组比较:染毒36h后NiSO450μmol/L组开始出现-OH·含量升高及T-AOC水平的下降;染毒各时间段,NiSO4200、400和800μmol/L组T-AOC、-OH·含量及其GSH-Px活力与对照组比较差异均有统计学意义(P<0.05)。相同浓度各染毒时间组与0h比较:NiSO450μmol/L染毒36h,开始出现T-AOC、-OH·含量的异常变化(P<0.05);NiSO4100μmol/L组染毒24h及其以上均出现GSH-Px、-OH·和T-AOC的改变(P<0.05);NiSO4200μmol/L及其以上组,各时间组T-AOC、-OH·、GSH-Px与0h比较差异均有统计学意义(P<0.05)。结论在本试验条件下,镍对小鼠精原细胞的损伤可能与其所致氧化应激效应增强有关。
Objective To explore the oxidative stress effect on mice spermatogonia induced by nickel sulfate（NiSO4） in vitro.Methods The single cell suspension was prepared with mice testes of birth 6-8 days and the cells were cultured routinely in order to acquire the purification spermatogonia.The cultured spermatogonia were administrated with nickel sulfate at the concentrations of 50,100,200,400,800 μmol/L and the control group treated with culture solution with same volume.After exposure 6,12,24,36 hours,the spermatigonia were harvested to detect the activities of total antioxidant capacity（T-AOC） and glutathione peroxidase（GSH-Px）,and the content of hydroxyl free radical（-OH·） with Microplate Reader.Results Compared with control group and 0 h groups,the content of -OH · increased and the activity of T-AOC decreased at exposure 36 h with NiSO4 50 mmol/L,the activity of GSH-Px was inhibited by NiSO4100 mmol/L at exposure 24 h in spermatogonia（P0.05）.At all exposure times,3 indexes of oxidative stress shown up abnormal changes at following concentrations of NiSO4200,400 and 800 μmol/L（P0.05）.Conclusion The result showed that the damage of mice spermatogonia was related to the enhancement of oxidative stress caused by nickel sulfate.
出处
《毒理学杂志》
CAS
CSCD
北大核心
2010年第1期34-37,共4页
Journal of Toxicology
基金
兰州大学医学科研基金项目资助(LZUYX200710)
兰州大学本科生教学质量工程项目资助
关键词
硫酸镍
精原细胞
氧化应激
Nickel sulfate
Spermatogonia
Oxidative stress
