摘要
目的探讨化学性低氧模拟剂氯化钴对人皮肤角质形成细胞(HaCat)炎症反应的影响。方法用不同浓度的CoCl2处理HaCat细胞,建立化学性低氧诱导皮肤细胞损伤的实验模型后,检测细胞存活率、细胞内活性氧(ROS)、线粒体膜电位(MMP)、细胞培养液中白介素6(IL-6)和白介素8(IL-8)水平以及血红素加氧酶(HO-1)表达。结果在500～3000μmol.L-1浓度范围内,CoCl2可降低HaCat细胞存活率,且CoCl2剂量越大、细胞存活率降低越明显;2000μmol.L-1CoCl2能诱导HaCat细胞产生氧化应激反应,使胞内ROS生成增多,MMP降低;CoCl2能诱导HaCat细胞产生炎症反应,使IL-6和IL-8释放增多;1000～3000μmol.L-1CoCl2能上调HO-1的表达。结论CoCl2在诱导HaCat细胞产生氧化应激反应的同时,也能引起炎症反应,促进IL-6和IL-8的释放及HO-1表达上调。
Aim To explore the effect of chemical hypoxia-mimetic agent,cobalt chloride（CoCl2）on inflammatory reaction in human keratinocytes（HaCat cells）.Methods After HaCat cells were treated with CoCl2 at different concentrations to set up a chemical hypoxia-induced cell model of skin injury,cell viability,intracellular reactive oxygen species（ROS）,mitochondrial membrane potential（MMP）,the levels of both interleukin 6（IL-6）and interleukin 8（IL-8）as well as the expression of heme oxygenase-1（HO-1）were detected.Results The viability of HaCat cells was reduced by CoCl2 at the concentrations from 500 to 3 000 μmol·L^-1,and the higher CoCl2 doses,the lower cell viability was.CoCl2 induced oxidative stress reaction（increasing ROS production and decreasing MMP）.CoCl2 induced inflammatory reaction,enhancing the release of IL-6 and IL-8.CoCl2 at concentrations from 1 000 to 3 000 μmol·L^-1 upregulated HO-1 expression in HaCat cells.Conclusion CoCl2 induces not only oxidative stress,but also inflammatory reaction,increasing the release of both IL-6 and IL-8,as well as HO-1 expression.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2010年第5期633-637,共5页
Chinese Pharmacological Bulletin
基金
广东省科技计划资助项目(No2008B080703053)
