摘要
目的:探讨自制中药提取物对人瘢痕疙瘩成纤维细胞(KFB)增殖的影响。方法:在体外培养的人KFB、正常皮肤成纤维细胞(NFB)培养液中加入不同浓度的自制中药水提物、醇提物,采用倒置显微镜、CCK-8试剂盒及生化分析仪对成纤维细胞的形态、增殖活性、生长状况及乳酸脱氢酶(LDH)活性进行观察与测定。结果:在一定浓度中药提取物的作用下,KFB形态发生改变,增殖活性降低,LDH活性增高;其中醇提物抑制KFB增殖较水提物更有效,KFB对中药的抑制反应比NFB更敏感。结论:自制中药可能通过抑制KFB增殖、细胞毒性作用等而发挥治疗瘢痕疙瘩的作用。
Objective To study the effects of the extractant from the self-made traditional Chinese drug on the proliferation of keloid-derived fibroblasts（KFB）.Methods KFB and normal fibroblasts（NFB） were cultured in vitro.The aqueous or alcohol extractant in various concentr ation from the self-made traditional Chinese drug was added to the culture media.Inverted microscope,CCK-8 and biochemistry analysator were applied to observe or detect fibroblasts appearance,proliferation,growth and lactate dehydrogenase（LDH） activity.Results Within certain concentration,after the extractant from drug taking action,KFB appearance was altered,the proliferative activity was decreased,the LDH activity was increased.In addition,the alcohol extractant inhibited the proliferation of KFB more efficiently than the aqueous extractant,KFB were more sensitive to drug than NFB.Conclusions The self-made traditional Chinese drug may produce a therapeutic effect by inhibiting the proliferation of KFB,cytotoxicity,etc.
出处
《中国美容医学》
CAS
2010年第5期701-703,共3页
Chinese Journal of Aesthetic Medicine
关键词
瘢痕疙瘩
中医药
成纤维细胞
keloid
traditional Chinese drug
fibroblast
