摘要
目的应用基因芯片研究大鼠肝缺血预处理后残存肝组织再生过程中基因表达谱的动态变化。方法 Sprague-dawley(SD)大鼠肝在缺血再灌注前行缺血预处理(10min缺血后10min再灌注),再灌注期间行70%肝叶切除建立余肝再生模型,用affmetrix RAT GeneArray 1.0ST基因表达谱芯片筛选大鼠再生肝组织中差异表达基因进行功能分析及归类。结果再生肝组织有差异表达基因1103条,涉及代谢相关基因、细胞周期调控基因、炎症反应相关基因、凋亡相关基因、信号传导相关基因、细胞因子相关基因、生长因子基因等,差异表达基因显著性的表达趋势有7种。结论缺血预处理后肝再生的过程是多基因调控的动态变化过程,用基因芯片有助于研究肝再生的机制,为促进肝再生提供治疗的潜在靶点。
Objective The aim of the research was to study the gene expression profile in liver regenerated from major hepatectomy after ischemie preconditioning （IPC） in rats. Methods Sprague-dawley （SD） rats were subjected to 30 min of total hepatic ischemia after ischemic preconditioning （ 10 min of ischemia, followed by 10 min of reperusion） and 70% hepatectomy was performed just before reperfusion and at 4, 24, 48 h after operation the total RNA was isolated from liver tissue of rats. The differentially expressed genes during different stages of liver regeneration were detected through affmetrix RAT GeneArray 1.0 ST gene chip technique and their functions were analyzed. Results During the process of liver regeneration liver tissue with a total of 1103 differentially expressed genes related to metabolism related genes,mitosis,cell cycle regulation genes,inflammation-related genes,apoptosis- related genes, signal transduction-related genes, cytokine-related genes, growth factor genesand so on. There are 7 kinds of expression trends. Conclusions The gcnome-wide changes in the expression profile in liver regenerated from major hepateetomy after ischemie preconditioning （IPC）in rats is complicated.Analyzing the changes in the gene expression by DNA chips can reveal the mechanism and may identify some drug targets for the treatment.
出处
《热带医学杂志》
CAS
2010年第4期410-414,共5页
Journal of Tropical Medicine
关键词
肝脏
预处理
肝再生
大鼠
基因芯片
liver
ischemie preeonditioning（IPC）
liver regeneration
rat
gene chip
