摘要
利用韭菜青×IR26杂交后代建立一个重组自交系群体(recombinant inbred line,RIL)及相应的SSR分子标记连锁图谱,在2007和2008年进行Cd2+(5 mg.kg-1)胁迫下水稻幼苗耐Cd2+胁迫的QTL分析。结果表明:2007年检测到3个与幼苗耐Cd2+胁迫有关的QTLs,包括1个控制相对苗高的位点qRSH-7和2个控制单株相对干重的位点qRDW-11a和qRDW-11b,分别位于第7和第11染色体;2008年检测到6个与幼苗耐Cd2+胁迫相关的QTLs,包括3个控制单株相对干重的位点qRDW-1、qRDW-2和qRDW-7,1个控制相对苗高的位点qRSH-2,2个控制相对鲜重的位点qRFW-2和qRFW-7,分别位于第1、2、7染色体。在定位的9个QTLs中,qRDW-7和qRFW-7都位于第7染色体的RM6872与RM11标记之间,加性效应分别为4.89和5.44,表型贡献率为18.02%和15.24%;qRSH-7和qRDW-1的加性效应分别为5.09和-3.64,表型贡献率为13.48%和10.06%;其余位点表型贡献率均低于10.0%。
The recombinant inbred line（RIL） populations derived from Jiucaiqing×IR26 and their genetic linkage maps were employed to map QTL controlling tolerance to Cd2＋ stress at rice seedlings with 5 mg·kg-1 by using the relative root length,relative seedling height,relative dry weight per plant and relative fresh weight per plant.The results showed that 3 QTLs on chromosome 7 and 11 were detected based on relative seedling height and relative dry weight per plant in 2007,i.e.qRSH-7,qRDW-11a and qRDW-11b.6 QTLs on chromosome 1,2 and 7 were detected based on relative root length,relative seedling height,relative dry weight per plant and relative fresh weight per plant in 2008,i.e.qRDW-1,qRSH-2,qRFW-2,qRFW-7,qRDW-2 and qRDW-7.Among the 9 QTLs,qRDW-7 and qRFW-7,on chromosome 7,explained 18.02% and 15.24% of the total phenotypic variation,and the additive effects of those QTLs were 4.89 and 5.44 respectively.qRSH-7 and qRDW-1 explained 13.48% and 10.06% of the total phenotypic variation,and the additive effects of those QTLs were 5.09 and-3.64 respectively.The other QTLs explained below 10.0% of the total phenotypic variation respectively.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2010年第3期1-5,共5页
Journal of Nanjing Agricultural University
基金
江苏省农业三项工程项目(sx(2006)123)
