摘要
目的建立莲芝消炎片质量标准。方法采用薄层色谱(TLC)法对莲芝消炎片中穿心莲、山芝麻进行定性鉴别;采用高效液相色谱(HPLC)法测定制剂中穿心莲内酯、脱水穿心莲内酯的含量。结果 TLC鉴别的色谱斑点清晰,阴性对照无干扰;穿心莲内酯进样量在0.08271～0.82710μg范围内呈良好的线性关系,r=0.9993;脱水穿心莲内酯进样量在0.2417～2.4170μg范围内有良好的线性关系,r=0.9998;穿心莲内酯平均加样回收率为97.8%,RSD=1.03%;脱水穿心莲内酯平均加样回收率为96.7%,RSD=1.14%。结论所建立的鉴别方法专属性强,定量方法简便、准确,能有效控制该制剂的质量。
Objective To establish the quality standard of lianzhi xiaoyan tablet. Methods Herba andrographitis,screwtree were identified by TLC,and the contents of andrographolide and dehydroandrographolide were determined by HPLC. Results The identification by TLC was distinct and clearly. The qualititative evaluation of andrographolide and dehydroandrographolide had a good linearity over the range of 0.082 71-0.827 10 μg,r=0.999 3; 0.241 7-2.417 μg,r=0.999 8,respectively. The average recovery of andrographolide and dehydroandrographolide was 97.8%,RSD=1.03% and 96.7%,RSD=1.14%,respectively. Conclusion The identification and determination methods established were specific,simple,accurate and reliable,and can be used to control the quality of lianzhi xiaoyan tablet effectively.
出处
《医药导报》
CAS
2010年第6期780-782,共3页
Herald of Medicine
