摘要
采用反相高效液相色谱法（ＲＰＨＰＬＣ）测定胰岛素聚乳酸微球的含量。在ＯＤＳ柱上，以乙腈０．０１％三氟乙酸（３０∶７０）为流动相，于２７６ｎｍ处进行检测。方法的平均回收率为９９．９３％，ＲＳＤ＝０．２４％（ｎ＝３）。线性范围在０．１０６４～２．１２８ｍｇ／ｍｌ。
RP HPLC analysis of insulin in poly D,L lactic acid (PDLA) microspheres was presented in this study. Separation and determination of insulin in PDLA microspheres were achieved on a Shim pack CLC ODS column (150 mm×6 mm ID, maintained at room temperature) with detection wavelength of 276 nm. The composition of the mobile phase was acetonitrile and 0.01% trifuroacetic acid (30∶70). Good linearity was obtained from 0.1064 to 2.128 mg/ml of insulin with C =4.491×10 -4 A -2.461×10 2( r =0.9999). The average recovery was 99.93%, with RSD of 0.24% ( n =3).
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1999年第1期24-26,共3页
Journal of China Pharmaceutical University
