摘要
以引进的俄罗斯优良的新西伯利亚银白杨(Populus bachofenii×P.pyram idalis,ROZ)杂交无性系组培不定芽为材料,研究了基本培养基、植物生长调节剂、蔗糖质量浓度、琼脂质量浓度和pH值对新西伯利亚银白杨组培不定芽生根率和根系质量的影响。结果表明:基本培养基、植物生长调节剂、蔗糖质量浓度、琼脂质量浓度和pH值对新西伯利亚银白杨组培不定芽生根率没有显著的影响,而对平均生根数、平均根长和偏根率有显著的影响。隶属函数综合分析表明:以WPM培养基、IBA 0.05 mg.L-1、蔗糖25 g.L-1、琼脂7 g.L-1和pH值为5.8时,新西伯利亚银白杨组培苗生根率可以达到98%,根系质量最好(生根数多、根长长、偏根率低)。
An experiment was conducted to study the effects of basic culture media,plant growth regulators,concentrations of sugar and agar,and pH value on rooting percentage and rooting quality of tissue-cultured shoots of Populus bachofenii × P.pyramidalis Roz.,a superior clone introduced from Russia.Results show that the basic culture media,plant growth regulators,concentrations of sugar and agar,and pH value have no significant effect on rooting percentage,but have significant effects on average rooting number,average root length,and one-side rooting percentage.Analysis of subordinate function values indicates that the tissue-cultured shoots on WPM medium supplemented with 0.50mg·L-1 IBA,25g·L-1 sugar,and 7g·L-1 agar manifest higher rooting number,longer root length and lower one-side rooting percentage when the pH of the culture medium is adjusted to 5.8 before autoclaving,with a rooting percentage of 98%.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2010年第1期20-23,共4页
Journal of Northeast Forestry University
基金
国家林业局"948"引进项目(2004-4-03)
关键词
新西伯利亚银白杨
组织培养
生根
根系质量
Populus bachofenii×P.pyramidalis
Tissue culture
Rooting
Root quality
