摘要
目的:研究人源抗菌肽LL-37和IL-8在慢性鼻窦炎鼻息肉组织中的表达。方法:采用半定量RT-PCR和免疫组织化学方法检测31例慢性鼻窦炎息肉组织中LL-37和IL-8的表达,并以11例慢性鼻炎下鼻甲组织作对照。结果:LL-37和IL-8 mRNA在所有息肉组织和下鼻甲组织中均呈阳性表达,二者在息肉组织中的表达均明显高于下鼻甲组织(均P<0.01),且在息肉组织中的表达呈正相关(P<0.01);LL-37与IL-8蛋白在鼻息肉组织中的阳性表达率均明显高于下鼻甲组织(均P<0.01),二者在息肉组织中的阳性表达率呈正相关(P<0.01)。结论:LL-37和IL-8可能在慢性鼻窦炎的发病中发挥一定作用;除天然免疫作用外,LL-37还可能通过增强获得性免疫提高机体的抗感染功能。
Objective：To investigate the expressions of LL-37 and IL-8 in chronic sinusitis with nasal polyps.Method：Semi-quantitative reverse transcription polymerase chain reaction（RT-PCR） and immunohistochemical staining were used to detect the expressions of LL-37 and IL-8 in nasal polyp tissues of 31 patients with chronic sinusitis and inferior turbinate tissues of 11 patients with chronic rhinitis.Result：LL-37 and IL-8 mRNA were all positively expressed in all nasal polyps and inferior turbinate tissues.There were significant increases of LL-37 and IL-8 mRNA expressions in nasal polyps compared with the inferior turbinate tissues（P〈0.01）.There were also significant increases of positive expression rates of LL-37 and IL-8 protein in nasal polyps,compared with the inferior turbinate tissues（P〈0.01）.There was a positive relationship between the mRNA and protein expressions of LL37 and IL-8（P〈0.01）.Conclusion：The expressions of LL-37 and IL-8 in nasal polyps suggest that they may play a role in the pathogenesis of chronic sinusitis.Besides its innate immune,LL-37 could enhance human body＇s anti-infected function by increasing acquired immune.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2010年第8期337-340,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
湖南省科技厅计划基金(No:06FJ3166)
