期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

磷酸化钙/钙调素依赖性蛋白激酶Ⅱ在胫骨癌痛大鼠脊髓背角中的表达变化 被引量：1

Expression Changes of pCaMKⅡin Spinal Dorsal Horn in a Rat Model of Bone Cancer Pain
原文传递
导出
摘要 目的观察胫骨癌痛模型大鼠脊髓背角磷酸化钙/钙调素依赖性蛋白激酶Ⅱ(pCaMKⅡ)的表达变化,探讨CaMKⅡ在骨癌痛中的作用。方法选择雌性SD大鼠54只,随机分为3组(n=18):空白组(N),不作任何处理;对照组(C),左侧胫骨上段骨髓腔注入5μlHank’s液;模型组(M),左侧胫骨上段骨髓腔注入5μlWalker256细胞(2×107/ml)。分别于建模前及建模后6d、12d、18d测定大鼠机械缩足反射阈值和双后肢负重差值(n=6),并在相应时间点随机取6只大鼠脊髓L4～5段,采用免疫组织化学法检测pCaMKⅡ的表达。结果与N组、C组比较,建模后6～18dM组大鼠出现进行性加重的疼痛行为学改变,脊髓背角pCaMKⅡ表达逐渐升高(P<0.05)。结论脊髓pCaMKⅡ表达上调可能参与了大鼠骨癌痛的产生和维持。 Objective To observe the changes of pCaMKⅡexpression in the spinal dorsal horn in a rat model of bone cancer pain.Methods Fifty-four rats were randomly divided into three groups （n= 18）：the normol group,without any operation;the control group,injecting Hank’s 5μl into tibia medullary cavity;the model group,injecting Walker256 cells （2×107/ml）5 μl into tibia medullary cavity.Response of the injuried hind paw to mechanical stimulation with von Frey filament and the difference of weight bearing between both hind paws were measured before and 6 d、12 d and 18 d after establishing model.Then 6 rats in each group were killed 6 d、12 d and 18 d after establishing model separately and lumbar 4-5 spinal cord was removed for detection of pCaMKⅡby immunohistochemical study.Results Compared with the normal and contnol group,model group displayed gradul develepment of pain behaviors during 6-18 days after establishing model：And the expression of pCaMK-Ⅱ in spinal dorsal horn was up-regulated progressively.An up-regulated expression of pCaMKⅡ in spinal dorsal horn suggesting pCaMKⅡ may contribute to bone cancer pain.Conclusion Bone cancer pain results in an up-regulation in the expression of pCaMKⅡ in spinal dorsal horn,suggesting pCaMKⅡ may contribute to bone cancer pain.
作者 周静 杨建平 任春光 李伟 王丽娜 许期年 王秀云 左剑玲
机构地区 苏州大学附属第一医院麻醉科 苏州大学附属第一医院脑神经研究室
出处 《苏州大学学报（医学版）》 CAS 北大核心 2010年第2期233-235,244,共4页 Suzhou University Journal of Medical Science
基金 国家自然科学基金资助课题(30872442) 江苏省卫生厅基金资助课题(H200855)
关键词 骨癌痛 钙/钙调素依赖性蛋白激酶Ⅱ 疼痛 脊髓 大鼠 bone cancer pain calcium/calmodulin dependent protein kinaseⅡ pain spinal cord rats
分类号 R738.106 [医药卫生—肿瘤]
  • 相关文献

参考文献9

  • 1Dai Y, Wang H , Ogawa A, et al. Ca^2+/calmodulin-dependent protein kinase Ⅱ in the spinal cord contributes to neuropathic pain in a rat model of mononeuropathy [ J]. Eur J Neurosei, 2005,21 ( 9 ) : 2467 - 2474.
  • 2Bruggemann I, Schulz S, Wibomy D, et al. Colocalization of the π-opioid receptor and Calcium/calmodulin-dependent kinase Ⅱ in distinct pain-processing brain regions[ J ]. Brain Res Mol Brain Res,2000,85(1/2) : 239 -250.
  • 3Fang L, Wu J, Lin Q, et al. Calcium-calmodulin-dependent protein kinase Ⅱ contributes to spinal cord central sensitization[ J]. J Neurosci ,2002,22(10) :4196 -4204.
  • 4胡计嬅,杨建平,刘磊,李彩芳,王丽娜.趋化因子Fractalkine在骨癌痛大鼠中的作用及其脊髓机制[J].苏州大学学报（医学版）,2008,28(6):897-899. 被引量：3
  • 5Galana A, Laird JM, Cervero F, et al. In vivo recruitment by painful stimuli of AMPA receptor subunits to the plasma membrane of spinal cord neurons [ J ]. Pain, 2004, 112 (3) :315 -323.
  • 6Toni LJ, Adam CL, Linda SS. Secondary hyperalgesia in the postoperative pain Model is dependent on spinal calcium/ealmodulin dependent protein kinase Ⅱα activation[J].Anesth Analg ,2007, 105 (6) : 1650 - 6.
  • 7Takako M, Vjekoslav M. Multiple kinase pathways mediate the early sciatic ligation-associated activation of CREB in the rat spinal dorsal horn [J]. Neurosci Lett, 2005,381 (1/2) : 80 -85.
  • 8Seong SC, Young JS, Eon JS, et al. Involvement of phosphorylated Ca^2+/calmodulin-dependent protein kinase Ⅱ and phosphorylated extracellular signal-regulated protein in the mouse formalin pain model[ J ]. Brain Res, 2006,1108 (1): 28-38.
  • 9Chen GD, Peng ML, Wang PY, et al. Calcium/calmodulin-dependent kinase Ⅱ mediates NO-elicited PKG activation to participate in spinal reflex potentiation in anesthetized rats [ J ]. Am J Physiol Regul Integr Comp Physiol, 2008,294(2) : 487 -493.

二级参考文献13

  • 1杨建平,蒋豪.大鼠蛛网膜下腔埋管并长期留置操作的改进[J].中华麻醉学杂志,1993,13(2):110-112. 被引量：123
  • 2Watkins LR, Milligan ED, Maier SF. Glial activation: a driving force for pathological pain [J]. Trends Neurosci 2001, 24(8):450-455.
  • 3McMahon SB, Cafferty WB, Marchand F. Immune and glial cell factors as pain mediators and modulators[J]. Exp Neurol, 2005, 192(2):444-462.
  • 4Verge GM, Milligan ED, Maier SF, et al. Fractalkine (CX3CL1) and fractalkine receptor (CX3CR1) distribution in spinal cord and dorsal root ganglia (DRG) under basal versus neuropathic pain conditions[J]. Eur J Neurosci, 2004,20(5): 1150-1160.
  • 5Miltigan ED, Zapata V, ChacurM, etal. Evidence that exogenous and endogenous fractalkine can induce spinal no- ciceptivefacilitation in rats [J]. Eur J Neurosci, 2004, 20 (9):2294-2302.
  • 6Hughes PM, Batham MS, Frentzel S, et ol. Expression of fractalkine (CX3CL1) and its receptor (CX3CR1), during acute and chronic inflammation in the rodent CNS[J]. Glia, 2002,37(4):314-327.
  • 7Jung S, Aliberti J, Graemmel P, et al. Analysis of fractalkine receptor CX3CR1 function by targeted deletionand green fluorescent protein reporter gene insertion [J]. Mol Cell Biol, 2000,20(11): 4106-4114.
  • 8Chapman GA, Moores K, Harrison D, et al. Fractalkine cleavage from neuronal membranes represents an acute event in the inflammatory response to excitotoxic brain damage[J]. J Neurosci, 2000,20:87.
  • 9Zhang RX, Liu B, Wang L, et al. Spinal glial activation in a new rat model of bone cancer pain produced by prostate cancer cell inoculation of the tibia[J]. Pain, 2005, 118(1-2): 125-136.
  • 10Milligan E, Zapata V, Schoeniger D, et al. An initial investigation of spinal mechanisms underlying pain enhancement induced by fractalkine, a neuronally released chemokine [J]. Eur J Neurosci, 2005,22(11):2775-2782.

共引文献2

同被引文献24

  • 1宋雪松,徐艳冰,曹君利,何建华,张励才,曾因明.cAMP反应元件结合蛋白介导磷酸化细胞外信号调节激酶在神经病理性痛形成中的作用[J].生理学报,2005,57(2):139-146. 被引量：16
  • 2Schwei MJ, Honore P, Rogers SD, et al. Neuro- chemical and cellular reorganization of the spinal cord in a murine model of bone cancer pain. J Neurosci, 1999, 19 : 10886- 10897.
  • 3Clohisy DR, Ramnaraine ML, Scully S, et al. Os- teoproteger in inhibits tumor-induced osteoclasto- genesis and bone tumor grow th in osteopetrotic mice. J O rthop Res, 2000, 18 : 967 - 976.
  • 4Mantyh PW. Clohisy DR, KoltzenburgM, et al. Molecular mechanisms of cancer pain. Nat Rev Cancer, 2002, 2 : 201 - 209.
  • 5Schwei M J, Honore P, Rogers SD, et al. Neuro- chemical and cellular reorganization of the spinal cord in a murine model of bone cancer pain. J Ne- urosci 1999, 19 : 10886- 10897.
  • 6Luger NM, Mach DM, Sevcik, BA, et al. Bone cancer pa in: from model to mechanism to therapy. J Pain and Symptom Manag, 2005, 29 : S32 - 46.
  • 7Mantyh PW. A mechanism-based understanding of bone cancer pain. Novartis Found Symp, 2004, 261 : 194 - 214; discussion 214 - 219,256 - 261.
  • 8Olson TH, Riedl MS, Vulchanova L, et al. An acid sensing ion channel(ASIC)localizes to small prim- ary afferent neurons in rats. Neuroreport, 1998, 9 : 1109 - 1113.
  • 9Shinoda M, Ogino A, Ozaki N, et. al Involvement of TRPV1 in nociceptive behavior in a rat model of cancer pain. J Pain. 2008, 9 : 687 - 699.
  • 10Tong W, Wang W, Huang J, et .al Spinal high- mobility group box 1 contributes to mechanical al- lodynia in a rat model of bone cancer pain. Bio- chem Biophys Res Commun. 2010, 395 : 572- 576.

引证文献1

二级引证文献7

苏州大学学报（医学版）
2010年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部