摘要
本文旨在研究低盐度下饲料蛋白质和维生素B6水平对凡纳滨对虾生长、成活和肌肉中转氨酶活力的影响。根据凡纳滨对虾的营养需求,以不含维生素的酪蛋白为蛋白质源,配制成蛋白质水平为25.5%和40.8%的2种等能基础饲料,在基础饲料中分别添加0和200mg/kg的维生素B6配制成4种试验饲料。试验选用480尾平均体重为(0.0144±0.0047)g的凡纳滨对虾,随机分为4组,每组3个重复,每个重复40尾。试验期间盐度为3‰,试验期30d。结果表明,饲料中添加200mg/kg的维生素B6可以显著提高凡纳滨对虾的增重率、成活率、肥满度及谷草转氨酶和谷丙转氨酶活力(P<0.05);虽然25.5%和40.8%的饲料蛋白质水平对凡纳滨对虾增重率、肥满度及谷草转氨酶和谷丙转氨酶活力均无显著影响(P>0.05),但40.8%蛋白质组对虾的成活率显著高于25.5%蛋白质组(P<0.05)。双因素方差分析结果显示,饲料中维生素B6和蛋白质对低盐度下凡纳滨对虾各项测定指标均无显著交互作用(P>0.05)。结果提示,配制饲料时应同时满足凡纳滨对虾对蛋白质和维生素B6的营养需要量,期望只满足或提高其中的一种而节约另外一种营养素以获得凡纳滨对虾的最佳生长和成活率的方式是不可行的。
A feeding trial was conducted to investigate the effects of dietary protein and vitamin B6 levels on growth and amino transferase activities of juvenile Pacific white shrimp （Litopenaeus vannamei,L.vannamei）. Purified basal diets were formulated using vitamin-free casein as the protein source. Two levels （0 and 200 mg/kg diet） of vitamin B6 were added to the 2 basal diets which contained 25.5% and 40.8% dietary protein,respectively. Four hundred and eighty shrimp with an average body weight of （0.014 4±0.004 7） g were randomly allotted to 4 groups with 3 replicates of 40 shrimp. The trial lasted for 30 d and the salinity was 3‰. Results showed that supplementation of vitamin B6 200 mg/kg in diet could significantly increase the weight gain ratio （WGR）,survival rate （SR）,condition factor （CF） and activities of glutamic oxaloacetic transferase （GOT） and glutamic pyruvic transferase （GPT） of L.vannamei （P〈0.05）; the WGR,CF and activities of GOT and GPT of L.vannamei fed diets containing 25.5% and 40.8% protein had no significant differences （P〉0.05）,but SR in 40.8% protein group was significantly higher than that in 25.5% protein group. The Two-way ANOVA results showed that there was no significant interaction between dietary protein and vitamin B6 in all parameters of L.vannamei at low salinity （P〉0.05）. All results in this study indicated that dietary vitamin B6 and protein had different nutritional effects on L.vannamei,and when preparing diets for L.vannamei cultured at low salinity,dietary vitamin B6 and protein must meet the requirement of L.vannamei at the same time. It was infeasible to try to meet or increase one of those 2 nutrients to get the maximum growth and survival rate of L.vannamei with the aim to spare the other nutrient.
出处
《动物营养学报》
CAS
CSCD
北大核心
2010年第3期634-639,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
上海市2009自然科学基金项目(09ZR1409800)
高等学校博士点专项基金项目(200802690012)
上海市曙光跟踪计划项目(06GG06)
国家自然科学基金项目(30771670)
浙江省科技重大项目(2005C12006-01
2006C12005)
