摘要
目的 探讨跑台运动训练对局灶性脑缺血大鼠神经功能恢复和脑缺血组织中热休克蛋白70（HSP70）及C-MYC表达的影响.方法 将42只清洁级成年雄性SD大鼠分为假手术组6只、模型组18只、运动组18只.模型组、运动组大鼠采用改良的Longs线栓法制备大脑中动脉闭塞（MCAO）脑缺血模型,运动组于造模成功后24 h采用跑台训练器进行运动训练,每周6 d,共2周,其余2组则置于普通笼内饲养,期间可自由活动、进食.采用修正的神经行为学评分方法评价模型组和运动组大鼠MCAO脑缺血后第3,7,14天的神经功能,并断头取脑,采用逆转录-多聚酶链反应（RT-PCR）法、免疫组织化学和Western blot法检测脑缺血组织中HSP70、C-MYC的表达.结果 运动组大鼠脑缺血第7,14天神经功能评分均明显优于模型组（P〈0.05或0.01）;运动组大鼠脑缺血第7,14天HSF70和C-MYC表达较模型组增强（P〈0.05或0.01）.结论 运动训练可促进脑缺血大鼠神经功能恢复,其机制可能与上调脑缺血组织中HSP70及C-MYC表达有关.
Objective To observe the effects of treadmill training on the recovery of neurological function and the expression of HSP70 and C-MYC in the brains of rats with focal cerebral ischemia. Methods Forty-two male adult Sprague-Dawley rats were randomly divided into a sham group （ n =6）, a model group （n =18） and a treadmill exercise group （n=18）. Focal cerebral ischemia was induced by right middle cerebral artery occlusion （MCAO） in the model group and exercise group using a modified version of Longa＇s method. The rats in the treadmill exercise group were given treadmill training 6 d per week for 2 weeks after 24 h of MCAO. By contrast, the rats in the sham group and the model group were reared in standard cages. Before the rats were sacrificed at the 3rd, 7th and 14th d after MCAO, their neurological functions were tested using modified neurological severity scores （ mNSS） , and the mRNA and protein levels of HSP70 and C-MYC were detected using a reverse transcriptase-polymerase chain reaction （RT-PCR） , immunohistochemistry and Western blotting. Results Neurological function in the exercise group at the 7th and 14th days after MCAO had improved significantly compared with the control and model groups.The mRNA and protein levels of HSP70 and C-MYC were significantly upregulated at the 7th and 14th days. Conclusions Treadmill training can improve neurological function by upregulating the expression of HSP70 and C-MYC in the ischemic brain after MCAO.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2010年第5期333-337,共5页
Chinese Journal of Physical Medicine and Rehabilitation
基金
福建省卫生厅青年科研课题资助项目（20070227）,福建医科大学教授基金（JS06077）
关键词
脑缺血
康复训练
热休克蛋白70
C—MYC
Cerebral ischemia
Rehabilitative training
Heat shock protein 70
C-MYC
