摘要
单头实蝇高质量基因组DNA的获得为实蝇分子生态学研究奠定了重要基础。本文提出一种经济高效的实蝇基因组DNA提取方法,该方法广泛适用于不同虫态、不同保存条件的实蝇标本,与传统的CTAB法和SDS法相比操作简单、耗时短,得率高。
An effective method for extracting genomic DNA from individual specimens is important for the study of molecular phylogenitics in the fruit fly.This paper presented a method for extracting genomic DNA from individual fruit fly.The results showed that the genomic DNA could be successfully extracted from adult,larval and pupal of fruit flies.Meanwhile,this method is also suitable for dried specimens or specimens preserved in alcohol.Compared with traditional methods like CTAB and SDS,it is pretty simple,time saving and effective.
出处
《昆虫知识》
CSCD
北大核心
2010年第3期600-603,共4页
Entomological Knowledge
基金
国家质量监督检验检疫局重点项目(2008IK264)
