摘要
以三聚氰胺(MAM)为模板分子,甲基丙烯酸(MAA)为功能单体,二甲基丙烯酸乙二醇酯(EGDMA)为交联剂,原位聚合法制备了对MAM有很强选择性识别能力的分子印迹整体柱。采用脉冲洗脱法快速筛选MAM的洗脱剂,通过前沿色谱法测定了整体柱对MAM的结合容量。实验表明,所制备的印迹整体柱对MAM有极强亲和作用力,即使在强极性流动相中(甲醇或甲醇-水(V/V=80/20)),MAM在印迹柱上也有强保留,不被洗脱。实验测得结合位点数(Lt)和解离常数(Kd)分别为:印迹柱Lt=2.28×103μmol/g,Kd=2.45×10-5mol/L;空白柱Lt=366μmol/g,Kd=23.7mo/L。该印迹整体柱有望作为固相萃取柱,在线或离线选择性富集样品中的MAM。
A molecularly imprinted monolithic column with special molecule recognition ability for melamine（MAM） was prepared by in situ polymerization method as the HPLC stationary phase.MAM was used as template molecule,methacrylic acid（MAA） as functional monomer and ethylene glycol dimethacrylate（EDMA） as crosslinker during the polymerization process.Pulsed elution format was applied to rapid screening elution agent and frontal chromatography method also applied to investigate the binding capacities of imprinted and non-imprinted monoliths.The experimental results showed that the imprinted monolithic column possesses good affinity to MAM.MAM can be thoroughly reserved by the imprinted column.The numbers of binding sites and the dissociation constants were determined as 2.28 × 10^3 μmol /g and 2.45 × 10^-5 mol /L for imprinted column,366 μmol /g and 23.7mol /L for non-imprinted column,respectively.
出处
《化学通报》
CAS
CSCD
北大核心
2010年第6期545-550,共6页
Chemistry
基金
广东药学院博士启动项目(2006YKX01)
广东省科技计划项目(2009198)项目
关键词
分子印迹聚合物
整体柱
三聚氰胺
Molecularly imprinted polymer
Monolithic column
Melamine
