摘要
目的分析研究夫妻双方艾滋病毒（HIV）感染病毒株亚型特征和夫妻问传播关系。方法以浙江省艾滋病疫情报告中双方均感染HIV的夫妻为研究对象，基因序列分析结合个案危险行为史分析，采用巢式PCR扩增gag基因区，分析亚型特征、夫妻间基因距离，并做系统进化树分析夫妻间传播关系。结果获得46对夫妻（92例）的样本检测结果，其中39例CRF01_AE、10例CRF07_BC、18例CRF08_BC、18例B、5例C、2例CRF02_AG六种亚型，分别占42．4％、10．9％、19．6％、19．6％、5．4％和2．2％。夫妻双方亚型一致的为44对（95．7％）。经系统树分析，41对夫妻序列中，33对（80．5％）提示有夫妻间直接传播关系，其中CRF01_AE、CRF08_BC和CRF02_AG主要为非婚性行为感染后经婚内性行为传播，B亚型和CRF07BC主要为经血传播感染后经婚内性行为传播。结论浙江省夫妻双方感染HIV的病毒株亚型呈现多样性，CRF01_AE是主要类型。一方高危行为感染后经婚内性传播是夫妻人群感染的主要模式。
Objective To analyse the subtype and transmission of HIV strain in both HIV infected spouses. Methods Reported both HIV infected spouses were selected as objects. Analysis on genetic sequence and high risk behaviors was carried out. Subgenomic gag was amplified by nest-PCR analysis of whole blood samples from objects. Genetic subtype characterization of HIV was identified and pairwise genetic distances were calculated. Sequence relationships were also examined by phylogenetic tree analysis. Results Genetic sequences of 46 pairs （92 cases） were obtained. The genetic subtype comprised 39 CRF01_AE （42.4%）, 10 CRF07_BC （ 10.9% ）, 18 CRF08_BC （ 19.6% ）, 18B （19.6%） ,5C （5.4%） and 2 CRF02_AG（2.2%）. 44 pairs had the same subtype between the two partners, accounted for 95.7% of the total. 33 of the 41 pairs with phylogenetic tree analysis were found having epidemiological linkage in pair wise. Sexual behaviors of out-marriage were the main risk factors of CRF01 AE and CRF08 BC and CRF02 AG strains infection. Blood transmission was associated with B and CRF07 BC. Conclusion The HIV strains subtype detected in HIV infected spouses characterized with diversity. CRF01_AE was the main strain subtype. The main route of transmission to the spouses was through unprotected sexual contacts. Surveillance programs on HIV infected parmer together with intervention between the spouses should be improved.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2010年第6期638-641,共4页
Chinese Journal of Epidemiology
基金
浙江省医药卫生科学研究基金（2007A040）（感谢浙江省各市和相关县、市、区疾病预防控制中心的支持和协作）
关键词
艾滋病病毒
亚型
传播关系
夫妻
Human immunodeficiency virus
Genetic subtype
Transmission relationship
Spouses
