摘要
目的在体外培养条件下,探讨阿魏酸钠(SF)拮抗氧化低密度脂蛋白(Ox-LDL)的作用机制。方法在体外培养人脐静脉内皮细胞(HUVEC)中分别和同时加入SF和Ox-LDL,采用基因芯片技术分析内皮细胞中白细胞介素-1β(IL-1β)的基因表达改变;用实时PCR技术(RT-PCR)检测IL-1βmRNA表达,用酶联免疫吸附试验(ELISA)检测IL-1β蛋白表达。结果基因芯片检测结果显示,HUVEC受Ox-LDL诱导后,IL-1β基因表达上调,比空白对照组高2.4倍;而采用SF+Ox-LDL处理后,IL-1β基因表达下调,是Ox-LDL组的2.6倍。RT-PCR与ELISA结果显示,Ox-LDL组与空白对照组比较,IL-1βmRNA及蛋白表达均明显增高;SF+Ox-LDL组与空白对照组比较,IL-1βmRNA表达差异无统计学意义;蛋白表达则明显降低。结论结论 Ox-LDL可诱导内皮细胞的IL-1β表达明显增强,阿魏酸钠能够抑制Ox-LDL活性,下调IL-1β的mRNA表达,可减少IL-1β对血管内皮细胞的损害。
Objective To study the antagonistic effect of sodium ferulate（SF） on oxidized low-density lipoprotein（Ox-LDL） in human umbilical vein endothelial cells.Methods Human umbilical vein endothelial cells（HUVEC） were cultured in vitro.Gene chip technology was used to analyze the change of interleukin（IL）-1β gene expression in HUVEC after the treatments of Ox-LDL,SF＋Ox-LDL,respectively.The IL-1β mRNA was measured with real time PCR（RT-PCR） and IL-1β protein by enzyme-linked immunosorbent assay（ELISA）.Results The expression of IL-1β gene was up-regulated by 2.4 folds after the induction of Ox-LDL in HUVECs compared with that of the control.While the expression of IL-1β gene was down-regulated by 2.6 folds after the induction of SF＋Ox-LDL in HUVEC compared with that of in the group of Ox-LDL.The results of RT-PCR and ELISA showed that both the expression of IL-1β mRNA and IL-1β protein increased significantly in Ox-LDL group compared with that of the control.The expression of IL-1β protein decreased obviously while the expression of IL-1βmRNA didn’t change significantly in SF＋Ox-LDL group compared with that of the control.Conclusion Ox-LDL could induce the expression of IL-1β,which could be inhibited by sodium ferulate.Meanwhile,sodium ferulate could also down-regulate the expression of IL-1β so as to reduce the damage of IL-1β in endothelial cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第6期689-691,共3页
Chinese Journal of Public Health
基金
国家科技部项目(2005DFA30510)
