摘要
目的探讨逍遥散治疗慢性应激致肝郁脾虚证的作用机制。方法将100只雄性SD大鼠随机分为正常组、假手术组、模型组、逍遥散组、6-氰基-7-硝喹啉-2,3-双酮(CNQX)组和联合用药组。造模大鼠通过连续21 d慢性束缚应激建立肝郁脾虚证候模型,逍遥散组、联合用药组大鼠每天束缚前灌服逍遥散5.32 g/kg,CNQX组、联合用药组大鼠在第1、4、7、10、13、16、19、21天右侧杏仁核区微量注射α-氨基羟甲基恶唑丙酸(AMPA)受体拮抗剂CNQX 0.5μg。各组大鼠第22天进行旷场实验和高架十字迷宫实验。结果在旷场实验中,模型组大鼠的中央区停留时间明显增多,5 m in运动总长度明显减少。在高架十字迷宫实验中,模型组大鼠进入中央区的次数和停留时间、进入开放臂的次数和停留时间均明显减少;封闭臂停留时间明显增加。CNQX和逍遥散对旷场和高架十字迷宫各项指标的改善作用明显,二者联合作用效果较好。结论逍遥散可改善肝郁脾虚模型大鼠的行为学变化,其作用机制可能与CNQX一致。
Abstract： Objective To discuss the mechanism of Xiaoyao Powder in the treatment of liver depression and spleen deficiency syndrome induced by chronic stress. Methods All 100 male SD rats were randomly divided into the normal group, sham-operation group, model group, Xiaoyao Powder group, CNQX group and Xiaoyao Powder combining with CNQX group （combining group）. The model of liver depression and spleen deficiency syndrome was established in rats by chronic bondage stress for 21 days. The Xiaoyao Powder group and combining group were given Xiaoyao Powder （5.32 g/kg） orally every day before bondage. The CNQX group and combining group were injected with CNQX （a receptor antagonist of AMPA, 0.5 Ixg） in the right amygdale on the 1st, 4th , 7th , 10th , 13th, 16th＇ 19th and 21~t day, respectively. The open-field test and elevated plus-maze test were carried out in rats of all groups on the 22nd day. Results In the open-field test the stay period in the central zone increased significantly, and the total distance of 5-minute motion decreased significantly in the model group. In the elevated plus- maze test the times and stay period in the central zone, and times and stay period of entering open arm decreased significantly, and the stay period in close arm increased significantly in the model group. All indexes were improved significantly by CNQX or Xiaoyao Powder in the open-field test and elevated plusmaze test. Xiaoyao Powder combining with CNQX had better effect. Conclusion Xiaoyao Powder can relieve the ethologic changes of rat model of liver depression and spleen deficiency syndrome, and the mechanism is as the same as that of CNQX.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2010年第5期317-322,共6页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金项目(No.30672578)
国家杰出青年科学基金项目(No.30825046)
