摘要
建立了免疫亲和柱净化高效液相色谱法测定坚果中黄曲霉毒素B1,B2,G1和G2总量。样品用甲醇—水提取,经免疫亲和色谱柱纯化,应用液相色谱法检测。试验结果表明:空白样品分别按照5.2,26,52μg/kg添加黄曲霉毒素,回收率为81.3%～96.0%,精密度<10%,黄曲霉毒素B1,B2,G1和G2的检测灵敏度分别为0.10,0.05,0.10,0.15μg/kg。免疫亲和柱净化高效液相色谱法测定坚果中黄曲霉毒素B1,B2,G1,G2总量,是一种简单、快速和准确的方法。
A simple and rapid method for determination of aflatoxin B1,B2,G1 and G2 content in nut by immune affinity column cleanse by HPLC were developed. After extraction with MeOH-H2O,the extracts of nuts homogenization was loaded on the IAC column for purification and determined by HPLC.The recoveries of samples were in the range of 81.3%～96.0%,the RSD was below 10%. The limit of quantification for aflatoxin B1 was 0.1 μg/kg,the limit of quantification for aflatoxin B2 was 0.05 μg/kg,the limit of quantification for aflatoxin G1 was 0.1 μg/kg,the limit of quantification for aflatoxin G2 was 0.15 μg/kg. Determination aflatoxins B1,B2,G1 and G2 content in nut by immune affinity column was a rapid,simple and exact method.
出处
《农产品加工（下）》
2010年第6期32-34,共3页
Farm Products Processing
基金
国家质检总局科研计划项目(2008IK117)
