摘要
为了研究废弃农作物作为反硝化脱氮处理的新型碳源和生物膜载体中微生物的多样性及微生物的群落结构,将玉米芯作为反硝化碳源和生物膜的载体布置在河道中,采集同步脱氮过程中的玉米芯样品并提取微生物总DNA,使用细菌通用引物对(GC341F和518R)从总DNA中成功扩增出目标16SrDNA片段,然后对扩增的16SrDNA进行变性梯度凝胶电泳(DGGE)测定,对凝胶染色并进行条带统计分析和切胶测序。结果表明以玉米芯为载体的生物膜优势菌变化规律与生存环境的变化存在较好的相关性,当水体中溶解氧提高后,生物膜上的优势种群以好氧/兼氧的异养杆状细菌Bacillus为主。
In order to research the agriculture residues, the new carbon resources of nitrogen removal by denitrification, and the diversity and community structure of microorganism in biofilm carriers, I place some corncobs in the waterway as carbon sources and biofilm carriers, total microbial DNA was extracted from samples which were collected from the corncobs during synchronous nitrogen removal, 16SrDNA fragments were amplified from the total DNA successfully using a pair of universal bacterial 16SrDNA primer . GC341F and 518R , and then were used for denaturing gradient gel electrophoresis （DGGE） analysis . The bands in the gel were analyzed by statistical methods and excided from the gel for sequencing , and the sequences were used for homology analysis. It is indicated that the variation of the predominant bacteria on biofilms that takes corncobs as its carriers relates closely to the changes of its survival environment. When the dissolved oxygen amount increases in the water body, the predominant bacteria on biofilms is mainly aerophilic/facultative heterotrophy bacillus.
出处
《上海化工》
CAS
2010年第6期1-4,共4页
Shanghai Chemical Industry
