摘要
目的探讨蛋白酪氨酸磷酸酶1B(PTP-1B)基因位点IVS6+G82A多态性与高血压胰岛素抵抗中医证候特点的相关性。方法高血压患者116例,分为胰岛素抵抗组"(47例)和非胰岛素抵抗组(69例),同时选取正常体检者40名作为正常组,采用多聚酶联反应-限制性片段长度多态性法进行PTP-1B基因位点IVS6+G82A多态性分析。检测所有研究对象空腹血糖、空腹胰岛素(FINS),计算HOMA-IR,同时评估高血压患者阳亢证候积分。结果高血压胰岛素抵抗组PTP-1B基因IVS6+G82A位点GG基因型频率明显高于正常组及非胰岛素抵抗组(P<0.05),高血压患者不含A等位基因者(GG型)FINS、HOMA-IR及阳亢证候积分显著高于含A等位基因者(AA型+AG型,P<0.05)。结论 PTP-1B基因IVS6+G82A位点G突变与高血压胰岛素抵抗肝阳亢盛证候特点具有一定的相关性。
Objective To study the association of IVS6＋G82A variant in protein tyrosine phosphatase-1B（PTP-1B） with traditional Chinese medicine（TCM）syndrome characteristic in hypertensive patients（pts） with insulin resistance.Methods One hundred and twelve pts with hypertension and forty health controls were screened.Pts with hypertension were divided into insulin resistance group（n=47） and non insulin resistance group（n=69）.The PTP-1B IVS6＋G82A polymorphism was determined using polymerase chain reaction and restriction enzymes.Fasting blood glucose（FBG） and insulin were detected and the integrals of hyperactivity of liver-yang syndrome was evaluated.Insulin resistance was assessed by the homeostasis model assessment of IR（HOMA-IR） equation.Results The GG genotype frequency of IVS6＋G82A polymorphism in insulin resistance group were higher than that in non insulin resistance and control groups.in pts with hypertension,plasma fasting insulin,HOMA-IR and the integrals of hyperactivity of liver-yang syndrome in carriers of A allele（AA＋AG）were lower than those no carriers of A allele（GG）.Conclusion The polymorphism of IVS6＋G82A was associated with insulin resistance and characteristics of hyperactivity of liver-yang syndrome in hypertension.
出处
《中西医结合心脑血管病杂志》
2010年第6期646-648,共3页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
关键词
高血压
胰岛素抵抗
中医证候
蛋白酪氨酸磷酸酶
hypertension
insulin resistance
polymorphism
traditional Chinese medicine syndrome
protein tyrosine phosphatase-1B
