摘要
目的:观察氯化锰对大鼠睾丸间质细胞睾酮合成及去势大鼠生殖内分泌的影响,探讨氯化锰的抗雄激素样作用。方法:①Percoll梯度离心分离、纯化大鼠睾丸间质细胞,根据氯化锰染毒剂量分为对照(0mol/L氯化锰)和1.0×10-6、2.5×10-6、5.0×10-6、1.0×10-5、2.5×10-5、5.0×10-5及1.0×10-4mol/L氯化锰组,培养24h后,采用台盼蓝染色法测定大鼠睾丸间质细胞存活率。②同法分组,观察氯化锰对基础状态和人绒毛膜促性腺激素(HCG)刺激下睾丸间质细胞睾酮合成的影响。③将行睾丸摘除术的大鼠随机分为6组(n=10):溶剂对照组皮下注射玉米油0.2mL,阴性对照组皮下注射丙酸睾酮(TP)1.0μg,氯化锰低、中、高剂量组皮下注射1.0μgTP后分别注射氯化锰7.5、15.0和30.0mg/kg,阳性对照组皮下注射1.0μgTP后注射氟他胺(100.0mg/kg),1次/d,连续7d。7d后,用放射免疫法测定各组去势大鼠血清睾酮水平和前列腺特异抗原(PSA)含量,分离雄激素依赖组织,称量,计算脏器系数。结果:①随氯化锰染毒剂量的升高,大鼠睾丸间质细胞存活率逐渐下降(F=15.297,P=0.023)。②基础状态和HCG刺激下各组大鼠睾丸间质细胞睾酮水平差异均有统计学意义(F=32.639和25.187,P均<0.001);基础状态下氯化锰剂量≥5.0×10-6mol/L时睾酮水平均低于对照组(P<0.05),HCG刺激状态下氯化锰剂量≥1.0×10-5mol/L时睾酮水平低于对照组(P<0.05)。③各组去势大鼠血清睾酮、PSA含量、腹侧前列腺及精囊腺脏器系数相比,差异均有统计学意义(F=39.920,25.403,15.562和9.476,P均<0.05),但氯化锰低、中和高剂量组去势大鼠血清睾酮和PSA水平与阴性对照组比较,差异均无统计学意义(P均>0.05)。氯化锰高剂量组腹侧前列腺和精囊腺脏器系数均低于阴性对照组(P<0.05)。结论:氯化锰可能有抗雄激素样作用。
Aim：To study the effects of manganese chloride on testosterone biosynthesis in leydig cells from rat testes,and reproductive endocrine functions of castrated male rats.Methods：Leydig cells were isolated and allocated into control group,1.0×10-6,2.5×10-6,5.0×10-6,1.0×10-5,2.5×10-5,5.0×10-5,and 1.0×10-4 mol/L manganese chloride groups,respectively. The cell survival rate was observed by Trypan blue dye exclusion method. The leydig cells were allocated into 8 groups as above,and the testosterone level was measured when being stimulated by HCG or without HCG. By the Hershberger assay,the castrated SD rats were allocated into 6 groups,0.2 mL corn oil was administered in the solvent-control group,1.0 μg testosterone propionate （TP） was administered in the negative control group,manganese chloride exposure groups were given 7.5,15.0,and 30.0 mg/kg manganese chloride after injection of 1.0 μg TP,and the positive control group was given 1.0 μg TP and 100.0 mg/kg flutanide. After 7 days administration,the levels of testosterone and prostate specific antigen（PSA） in serum were detected using radioimmunoassay,and the tissues depended on androngen were weighed using analytic balance.Results：The survival rate of leydig cells decreased with increased of manganese chloride（F=15.297,P=0.023）.The testosterone level of the 8 groups had significant differences when being stimulated by HCG or without HCG（F=32.639 and 25.187,P0.001）. The testosterone level in manganese chloride exposure groups was lower than that of the control group when the dose of manganese chloride was higher than 5.0×10-6mol/L in basal state（P0.05）,which was lower than that of the control group when the dose of manganese chloride was higher than 1.0×10-5mol/L in HCG stimulated state（P0.05）.The serum levels of testosterone and PSA,and organ coefficients of ventro-prostate and seminal vesicle among the 6 groups had significant differences（F=39.920,25.403,15.562,and 9.476,P0.05）,while the serum levels of T and PSA of the rats administered manganese chloride had no significant difference from those of the negative control group （P0.05）. The organ coefficients of ventro-prostate and seminal vesicle of the rats administered manganese chloride at 30 mg/kg were lower than those of the negative control group（P0.05）.Conclusion：Manganese chloride may have antiandrogenic effects.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第3期419-422,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省自然科学基金资助项目0611045300
关键词
氯化锰
雄性大鼠
睾丸
间质细胞
抗雄激素
manganese chloride
male rat
testis
leydig cell
antiandrogen
