摘要
目的检测泌尿系统肿瘤多药耐药基因及其产物的表达。方法对肾颗粒细胞癌细胞系ＧＲＣ１，肾透明细胞癌细胞系ＲＣＣ９４９，膀胱移行细胞癌细胞系ＢＩＵ８７、Ｔ２４、ＥＪ，前列腺癌细胞系ＰＣ３ｍ和对照的白血病细胞系ＨＬ６０进行免疫细胞化学和逆转录聚合酶链反应（ＲＴＰＣＲ）检测。结果只有ＧＲＣ１同时表达Ｐ糖蛋白（ＰＧＰ）和ＭＤＲ１基因ｍＲＮＡ，其他肿瘤细胞系均无表达。结论泌尿系肿瘤细胞系多数无ＰＧＰ也没有ＭＤＲ１基因ｍＲＮＡ水平的表达。
Objective To determine the expression of MDR 1 gene and its product P glucoprotein in human renal cell carcinoma cell lines,bladder cacrinoma cell lines and prostate carcinoma cell line. Methods The study comprised of 2 renal cell carcinoma cell lines (GRC 1 and RCC 949),3 bladder carcinoma cell lines (BIU 87,T24 and EJ) and a prostate carcinoma cell line (PC 3m) with a negative comparative human leukemia cell line HL 60.The presence of MDR 1 mRNA was determined by RT PCR,and the P glucoprotein detected by immunocytochemical assay. Results The positive P glucoprotein could be only seen in a renal cell carcinoma cell line GRC 1,which was also the only cell line that the expression of MDR 1 mRNA could be found. Conclusions The renal cell carcinoma cell line GRC 1 expresses MDR 1 mRNA and its product P glucoprotein.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1999年第3期146-148,共3页
Chinese Journal of Urology
关键词
泌尿系肿瘤
多药耐药
逆转录
聚合酶链反应
Urinary neoplasms Multidrug resistance Reverse transcriptase polymerase chain reaction
