摘要
采用淀粉凝胶电泳技术对毛乌素沙地根茎灌木羊柴（ＨｅｄｙｓａｒｕｍｌａｅｖｅＭａｘｉｍ．）８个群体的遗传多样性和克隆结构进行了初步研究。利用１０个酶系统１５个等位酶位点的检测表明，羊柴群体具有较高的遗传变异水平，多态位点百分率Ｐ＝３７．０，等位基因平均数Ａ＝１．４８，平均期望杂合度Ｈｅ＝０．１０１；但８个群体间的分化很小（Ｆｓｔ＝０．０６７）；固定沙丘群体和半固定沙丘群体在等位酶水平上的变异性无显著差异。通过７个多态位点的研究表明，羊柴群体中的克隆多样性很高（Ｄ＝０．９１５６），但不同克隆在规模上相差很大。同时，群体间的克隆分化较大，广布基因型仅占３．２％。克隆空间结构的分析表明，羊柴的基株分布为游击型构型，克隆之间的镶嵌明显。
Genetic diversity and clonal structure of eight populations of Hedysarum laeve Maxim. in sandland were studied using starch gel electrophoresis. Allozyme data of 15 loci of 10 enzymes indicated relatively high levels of genetic variability in this species with a proportion of polymorphic 1oci P =37.0 and an average number of alleles per 1ocus of A =1.48 and an average expected heterozygosity of He =0.101. It exhibited a low degree of population differentiation among the eight populations with G ST value of 0.067. There was no significant difference of the genetic diversity between the populations in the fixed dunes and in the semi fixed dunes. The clonal diversity was very high ( D =0.9156) in H. laeve populations according to the analysis based on 7 polymorphic loci. The sizes of different clones, however, varied greatly. In addition, there was great clonal differentiation among populations with only 3.2% of the widespread genotyes. The analysis of clonal structure demonstrated that the spatial distribution pattern of H. laeve clones was one of the ‘guerilla’ architectures.
基金
中国科学院生物科学和技术研究特支费课题
关键词
羊柴
遗传多样性
克隆多样性
克隆结构
克隆植物
Hedysarum laeve , Genetic diversity, Clonal diversity, Clonal structure, Clonal plant
