摘要
背景:氟化物治疗骨质疏松一直备受争议,其对幼年大鼠的股骨生物力学参数影响的文献报道较少。目的:观察氟化钠对幼年大鼠股骨骨生物力学参数的影响。方法:SPF级的2月龄SD大鼠共90只,雌雄各半,被随机分成9组:对照组(幼年组、成年组、长期组)和用药组(幼年高氟组、幼年低氟组、成年高氟组、成年低氟组、幼年长期高氟组、幼年长期低氟组)。对照组灌胃生理盐水,用药组分别按相应时间给予不同剂量的氟化钠灌胃。实验结束后,大鼠被异戊巴比妥钠麻醉后,心脏取血处死大鼠。取大鼠左侧股骨进行三点弯曲力学试验,观察氟化物对幼年大鼠股骨最大载荷和刚度的影响。结果与结论:与幼年对照组相比,幼年高氟组股骨的最大载荷和刚度分别下降13.18%和13.61%(P<0.05),幼年低氟组的两项指标变化差异无显著性意义。与长期高氟组相比,幼年高氟组的最大载荷和刚度分别下降17.22%和17.17%(P<0.05);长期低氟组的最大载荷和刚度分别增加了18.33%和19.15%(P<0.05);成年高氟组和成年低氟组的最大载荷和刚度也有明显增加(P<0.05)。提示幼年大鼠对高剂量的氟化物更为敏感,高剂量的氟化物可降低大鼠的骨质量,随着氟化钠使用时间的延长,逐渐表现出对生长期大鼠骨质量的负作用。
BACKGROUND： Fluoride treatment of osteoporosis has been controversial. Literatures addressing the effect of fluoride on bone bio-mechanical parameters of femur in young rats are few. OBJECTIVE： To study the effects of fluoride on bone biomechanical parameters of femur in young rats. METHODS： Ninety 2-month-old SPF Sprague Dawley rats, half male and female, were randomly divided into 9 groups： control group （young, adult and long-time） and drug-administered group （young high-fluoride, young low-fluoride, adult high-fluoride, adult low-fluoride, long-term high-fluoride and long-term low-fluoride）. Rats in the control group were orally administered with physiological saline, while in the drug-administered group were given orally with different dose fluoride at the corresponding times. After experiment, rats were sacrificed under anaesthesia. Three-point bending test was performed at the left femur. The effects of fluoride on maximum load and rigidity of femur were measured. RESULTS AND CONCLUSION： Compared with young control group, the maximum load and the rigidity of femur in the young high-fluoride group were decreased by 13.18% and 13.61%, respectively （P 0.05）, which had no dramatically difference in the young low-fluoride group. Compared with long-term high-fluoride group, the maximum load and the rigidity of femur in the young high-fluoride were decreased by 17.22% and 17.17% （P 0.05）, which were obvious increased in the long-term low-fluoride group by 18.33% and 19.15%, respectively （P 0.05）. The maximum load and the rigidity of femur were strengthened in the adult high-fluoride and adult low-fluoride groups （P 0.05）. The results suggested that young rats are more sensitive to high-dose fluoride, which can reduce bone quality in rats. The negative effects on bone quality of rats were gradually displayed as the prolongation of the period of fluoride.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第11期1967-1970,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广东省自然科学基金资助项目06301445~~
