摘要
目的探讨小鼠皮肤切创愈合过程中损伤区M3受体的表达及其随时间的变化规律。方法应用免疫组织化学染色和Western印迹技术检测小鼠皮肤切创后不同时间段M3受体的变化情况。结果正常皮肤组织的表皮、毛囊、皮脂腺、汗腺、皮肌层等均表达M3受体。伤后6～12h,损伤区M3受体阳性细胞以多形核粒细胞为主;1～3d,M3受体阳性细胞以单个核细胞和成纤维细胞为主;5～14d,M3受体阳性细胞主要为成纤维细胞。阳性细胞率在伤后6～12h逐渐增高,12h达高峰,1～5d略有下降,但保持较高水平,7d阳性细胞率再次达到峰值,随后逐渐降低。通过Western印迹法检测显示,各个时间段均有M3受体阳性条带,其中12 h和7d为M3受体两个表达高峰。结论多形核粒细胞、单个核细胞和成纤维细胞均表达M3受体,提示其可能在皮肤切创愈合过程中起重要的作用;M3受体表达的规律性变化可用于损伤时间的推断。
Objective To investigate the expression of M3 subtype of muscarinic receptors（M3R） during the incised wound healing of the skin in mice and the characteristics of its time-dependent.Methods The change of M3R in skin incised wound was detected by immunohistochemical staining and Western blot.Results M3R-positive cells were detected in epidermis,hair follicle,sebaceous glands,sweat glands,dermomuscular layer in normal mouse skin.Expression of M3R was mainly detectable in polymorphonuclear cells（PMNs） in the wound specimens aged from 6 h to 12 h after injury.Afterwards,the M3R-positive cells were mostly mononuclear cells（MNCs） and fibroblastic cells（FBCs） at 1 d to 3 d post-injury,whereas the M3R-positive cells were mostly FBCs aged from 5 d to 14 d.Morphometrically,the ratio of the M3R-positive cells increased aged from 6 h to 12 h after injury,with a peak at 12 h.The ratios kept a high relatively level aged from 1 d to 5 d,but significantly that lowered as compared with aged 12 h after injury.The ratio reached the peak at 7 d again after injury,and then decreased gradually.The M3R protein also revealed a time-dependent tendency with double peaks at 12 h and 7 d after injury as detected by Western blotting.Conclusion M3R is time-dependently expression in PMNs,MNCs and FBCs suggesting that it may play roles during the skin incised wound healing,and M3R may be used as a marker for wound age determination.
出处
《法医学杂志》
CAS
CSCD
2010年第3期165-168,172,共5页
Journal of Forensic Medicine
基金
教育部高等学校博士学科点专项科研基金资助项目(200801590020)
