摘要
目的观察曲古抑菌素A(Trichostatin A,TSA)对甲状腺鳞癌SW579细胞株Akt、mTOR和p21基因表达的影响,探讨TSA抗甲状腺癌的作用机制。方法体外培养SW579细胞,用CCK-8法检测TSA对SW579的生长抑制作用,计算半数抑制浓度。实验分为对照组、TSA组、Wortmannin组、Wortmannin+TSA组、Rapamycin组,用RT-PCR方法检测SW579细胞Akt、p21及mTOR的mRNA表达,分析各处理组Akt、p21及mTOR基因表达变化。结果 CCK-8结果显示,TSA可明显抑制SW579的增殖,并呈剂量依赖性,半数抑制浓度约为147nmol/L。RT-PCR检测结果表明,与对照组相比,TSA组、Wortmannin组和Wortmannin+TSA组Akt、mTOR mRNA表达水平明显降低,TSA组p21表达显著上调,Wortmannin组p21表达明显降低,各组mTOR表达均明显降低。结论 TSA在一定浓度范围内对甲状腺鳞癌细胞株SW579有剂量依赖性的增殖抑制作用,其机制可能与TSA降低SW579细胞Akt、mTOR基因的表达,进而再上调p21的表达有关。
Objective To observe the effects of trichostatin A on the expression of Akt,p21 and mTOR in thyroid squamous cell carcinoma cell line （SW579） and to explore its mechanism.Methods SW579 cells were cultured in vitro. The antiproliferative effect of TSA against SW579 cells was tested by CCK-8 method,IC50 was calculated according to the growth inhibitory rates. SW579 cells were divided into control,TSA,Wortmannin,Wortmannin＋TSA and Rapamycin groups randomly. The Akt,p21 and mTOR mRNA expression levels were determined by RT-PCR. Analyze the changes of gene expression among each treatment group.Results The CCK-8 results showed that the proliferation index decreased markedly with a dose-dependent manner. The IC50 was about 147nmol/L. Compared with control group,the expression levels of Akt and mTOR mRNA were significantly lower in TSA,Wortmannin and Wortmannin＋TSA groups. The expression level of p21 mRNA was significantly higher in TSA group,but significantly lower in Wortmannin group. The expression level of mTOR mRNA was significantly lower in each group than in control group.Conclusions TSA can inhibit the proliferation of thyroid squamous cell carcinoma cell line with a dose-dependent manner,the mechanism of which may be related with decreasing of Akt and mTOR mRNA expression and increasing of p21 mRNA expression.
出处
《中国地方病防治》
2010年第3期173-176,共4页
Chinese Journal of Control of Endemic Diseases
基金
辽宁省自然科学基金(20062201)
辽宁省教育厅项目(05L132)
