摘要
本实验旨在探讨一种新的大鼠慢性哮喘模型建立方法。采用组胺激发对SD大鼠进行筛选,模型组经腹腔和皮下多点注射OVA(卵清白蛋白)致敏大鼠,1%OVA雾化激发1周建立急性模型,0.1%OVA雾化激发12周建立慢性模型,对照组以生理盐水代替。通过检测大鼠肺组织病理切片、BALF(肺泡灌洗液)炎性细胞构成、血清总IgE和OVA特异性IgE、肺功能等指标变化情况及被动皮肤过敏试验对模型进行评价,并对急、慢性模型进行了比较。结果显示,慢性哮喘模型组气道平滑肌及基底膜厚度、杯状细胞增生及胶原沉积情况、皮肤蓝斑溶出物光吸收度值、血清总IgE和OVA特异性IgE含量等指标均显著高于急性模型组,但气道炎性细胞浸润程度及BALF中嗜酸性粒细胞比例低于急性模型组;急、慢性模型组呼吸速率及呼气流量间无显著性差异;急、慢性哮喘模型组中以上各指标均显著高于正常对照组。因此,本实验成功制备大鼠慢性哮喘模型,基本复制了人哮喘病理生理特征。
This study is to establish a rat chronic asthma model.Sensitive SD rats were selected through histamine challenge.The asthmatic groups were sensitized by ih and ip with OVA,aluminium hydroxide gel and inactivated bacillus pertussis on day 1 and 14.From day 21,acute asthmatic group was aerosolized 1% OVA for 1 week,chronic asthmatic group was aerosolized 0.1% OVA for 12 weeks.The control groups received saline as the substitution of OVA.Twenty four hours after the last provocation,physiological monitoring equipment was used to detect the pulmonary function,then the rats were sacrificed.Bronchoalveolar lavage fluid（BALF） was collected to calculate the ratio of different inflammatory cells.ELISA was used to detect total IgE and OVA-specific IgE in serum.Microscopy was conducted to observe the histopathology of lung stained with haematoxylin and eosin staining.Collagen fibers were detected using Picric acid-Sirius red staining technique.The optical density at 610 nm of extractive from locus caeruleus was detected by passive cutaneous anaphylaxis（PCA）.The results showed that the asthmatic characteristics were significantly developed in model groups,but not in control groups.Chronic asthmatic group had significantly higher indexes than acute asthmatic group,including the thickness of airway smooth muscle and bronchial basement membrane,and goblet cell hyperplasia,the area of collagen in airways,A610 of extractive from locus caeruleus,the concentration of total IgE and OVA-specific IgE in serum.However,inflammatory cell infiltrate in lungs and the percentage of eosinophils of white blood cells in BALF were lower in chronic asthmatic group than those in acute asthmatic group.Respiratory rate and respiratory flow showed no significant difference in both model groups.In conclusion,the rat chronic asthma model is established by the way in this study,which is comparable to the physiopathologic characteristics of human asthma.
出处
《药学学报》
CAS
CSCD
北大核心
2010年第6期718-723,共6页
Acta Pharmaceutica Sinica
基金
河北省自然科学基金资助项目(C2010000248)
河北省教育厅项目(Z20090307)
河北大学引进人才项目(2008-140)
关键词
慢性哮喘
大鼠
气道重塑
chronic asthma
rat
airway remodelling
