摘要
从麻疯树胚乳cDNA文库中得到分子量大小约为17.5kDa的细胞质Ⅱ类小热激蛋白基因,命名为JcHSP17.5,GenBank登录号为GU320741。通过构建外源表达载体,将JcHSP17.5基因分别在大肠杆菌以及酿酒酵母中过量表达来研究基因的耐热和耐渗透压胁迫能力,发现两种重组菌在逆境下都较对照组生活力有显著提高。推测JcHSP17.5在麻疯树生长过程中可能与麻疯树耐热、耐旱以及耐离子胁迫相关。
JcHSP17.5,an approximately 17.5 kDa cytosolic class Ⅱ small heat shock protein（HSP） gene was cloned from a Jatropha curcas endosperm cDNA library（GenBank accession number is GU320741）.To verify its possible function,the thermally and osmotically tolerant characteristics were tested by overexpressing the recombinant JcHSP17.5 gene in Escherichia coli and Saccharomyces cerevisiae,respectively.The results showed that the recombinant cells exhibited significantly improved viability in both thermal and osmotic stress condition compared with the control.It suggested that the JcHSP17.5 represented an example of a HSP which was capable of protecting cells against environmental extreme.
出处
《植物生理学通讯》
CAS
CSCD
北大核心
2010年第5期441-447,共7页
Plant Physiology Communications
基金
"十一五"科技支撑项目(2006BAD07A04)
国家自然科技资源平台项目(2005DKA21403)
关键词
麻疯树
小热激蛋白
基因表达
耐热
耐渗透压
Jatropha curcas
small heat shock protein
gene expression
thermotolerance
osmotolerance
