摘要
利用PEG-CaCl2法将携带绿色荧光蛋白(sGFP)的表达载体pCT74转化进香蕉枯萎病菌4号生理小种(CGMCCC3.12196),sGFP在病原菌的菌丝及孢子的细胞质和细胞核内稳定表达。将sGFP标记的病原菌接种巴西蕉,证明sGFP的导入未引起病原菌致病性发生改变。水培9个枯萎病抗性级别不同的香蕉品种,分别收集根系分泌物,生物测定其对sGFP标记的病原菌的抑菌作用。荧光显微镜观察结果表明:高抗枯萎病的品种根系分泌物的有机成分对病原菌孢子有致死作用,中抗品种的对病原菌孢子萌发、菌丝的生长有显著抑制作用,相反感病品种的根系分泌物则能够起促进作用。
A green fluorescent protein （sGFP） tagged Foc4 strain was developed by the method of PEG-CaCl2.The fluorescent signal was clearly visible in cytoplasm and nucleus of the hyphae and spores under the fluorescence microscopy.The transformed Foc retained its pathogenicity and growth patterns.Water culture of 9 banana cultivars with different resistance were performed for 15 days using 200 mL of sterile distilled water for each plantlet,and the root exudates were collected and bioassay for their effects on the GFP tagged Foc4.The results showed that the apolar fraction of the root exudates from the highly resistant cultivars could kill the pathogen.Those of the general resistant cultivars could inhibit the spore germination and hyphal growth,while the sensitive cultivars couldn’t.
出处
《园艺学报》
CAS
CSCD
北大核心
2010年第5期713-720,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30971991)
国家科技攻关计划(2008BAD92B06)
农业部‘948’项目(2008-G1)
关键词
香蕉
香蕉枯萎病
绿色荧光蛋白
根系分泌物
抗病性鉴定
banana
fusarium wilt of banana
green fluorescent protein
root exudates
bioassay of resistance
