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hnRNP A2/B1 mRNA在非小细胞肺癌和转移淋巴结中表达及意义 被引量：6

hnRNP A2/B1 mRNA在非小细胞肺癌和转移淋巴结中表达及意义
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摘要 目的:探讨肺癌早期诊断基因核内不均一核糖体表达对肺癌淋巴结微转移的作用。观察hnRNPA2/B1在各组织类型肺癌中的表达。方法:42例非小细胞肺癌患者开胸手术纵隔淋巴结清扫获取原发灶及纵隔淋巴结病理标本,12例肺良性病变手术切除标本作正常对照。荧光定量PCR检测切除肿块和淋巴结的hnRNP A2/B1 mRNA表达。结果:(1)转移性淋巴结中hnRNP A2/B1 mRNA表达水平561.393显著高于无转移淋巴结156.669(P<0.05)。(2)Ⅲ期肺癌组织中hnRNP A2/B1 mRNA表达水平305(249,533)显著高于Ⅰ期和Ⅱ期肺癌组织(2=13.453,P<0.01)。(3)hnRNP A2/B1 mRNA在鳞癌和腺癌中的表达水平分别为207(152,305)和249(177,476),Z=-0.899,P>0.05。(4)58组淋巴结阳性,84组阴性,42个病人142组区域淋巴结hnRNP A2/B1mRNA阳性率为40.84%(58/142),12例肺良性疾病组织均阴性,HE染色42个病人的50组转移淋巴结阳性率为35.21%,与FQ-PCR 58枚(52.11%)无显著差异(P>0.05)。结论:NSCLC癌组织存在着hnRNP A2/B1的过度表达。NSCLC癌组织中hnRNP A2/B1表达水平与淋巴结转移状态及P-TNM分期有关。hnRNP A2/B1检验敏感性、特异性高,可用于肺癌的辅助诊断的标记。
作者 张洁霞 刘启才 何建行
机构地区 广州医学院第一附院肿瘤科 广州医学院分子生物学实验中心
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2010年第6期1221-1223,共3页 Chinese Journal of Pathophysiology
关键词 肺肿瘤 异质性胞核核糖核蛋白 微转移 Lung neoplasms Heterogeneous nuclear ribonucleoprotein Micro -metastasis
分类号 R73 [医药卫生—肿瘤]
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参考文献7

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共引文献6

同被引文献61

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引证文献6

二级引证文献18

中国病理生理杂志
2010年 第6期

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