摘要
目的探讨哈蟆油对D-半乳糖所致雄性衰老大鼠肝组织细胞周期素D1(CyclinD1)、周期性依赖性激酶6(CDK6)和细胞周期蛋白D/CDK激酶的抑制因子P15蛋白表达调控的影响。方法 SPF级SD雄性大鼠18只,随机分为模型、维生素E(VE)和哈蟆油组,每组6只,D-半乳糖颈背部皮下连续注射6周,另取雄性大鼠6只,同样部位每日注射生理盐水,作为空白组。第3周开始灌胃给药,给药时间30d。给药结束后收集标本采用Western blotting法检测肝组织CyclinD1、CDK6和P15蛋白的表达。结果与空白组比较,模型组大鼠肝脏CyclinD1蛋白、CDK6蛋白、P15蛋白表达明显下调。雄性衰老大鼠口服哈蟆油后,CyclinD1、CDK6和P15蛋白表达量(与内参比较)分别为41.73±0.54、23.29±0.30和21.49±0.30。与模型组相比,哈蟆油组能明显上调肝脏CyclinD1、CDK6、P15蛋白的表达(P<0.01)。结论哈蟆油能不同程度上调CyclinD1和CDK6蛋白表达,促进衰老雄性大鼠肝细胞增殖。同时反馈性上调细胞增殖负性调控蛋白P15表达,防止不必要的过度增殖。哈蟆油延缓雄性衰老作用可能通过调控肝脏有关增殖蛋白表达发挥抗衰老作用,与VE作用有所不同。
Objective To investigate the effect of Oviductus Ranae （OR） on the expressions of CyclinD1, CDK6 and P15 in the liver of aged male rats. Methods Eighteen male SD rats were randomly divided into 3 equal groups, namely the OR group, VE group and ageing model group. The rats received subcutaneous injection of D-galactose for 6 weeks to establish the aging models, and another 6 rats were injected daily with normal saline （NS） to serve as the normal control group. From the third week of the experiment, the rats were given oral OR or Vitamin E （VE） accordingly till the sixth week. After completion of the drug administration, all the rats were sacrificed for detecting the expressions of CyclinD1, CDK6 and P15 in the liver tissue by Western blotting. Results The relative expression levels of CyclinD1, CDK6 and P15 in the liver of the rats in the OR group were 41.73±0.54, 23.29±0.30 and 1.49±0.30, respectively, significantly up-regulated as compared with those in the ageing model group （P0.01）. The expressions of the proteins were obviously down-regulated in the model group in comparison with those in the normal control group. Conclusions OR treatment can lower the expressions of Cyclin D1 and CDK6 in the liver to enhance the liver cell proliferation in aged male rats. OR also promotes the expression of P15 through a feedback mechanism to prevent excessive proliferation of the cells. The effect of OR against ageing is mediated possibly by up-regulation of the proteins associated with the cell proliferation in the liver, a mechanism different from that of VE.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2010年第5期1044-1046,1049,共4页
Journal of Southern Medical University
基金
广东省科技计划项目(2006B35603002)
关键词
哈蟆油
衰老
肝脏
D-半乳糖
细胞增殖
Oviductus Ranae
aging
liver
D-galactose
cell proliferation
