摘要
目的观察验证尿苷三磷酸(UTP)对在体大鼠脑缺血再灌注损伤是否具有保护作用。方法线栓法制备大鼠中脑缺血再灌注(MCAO)损伤模型。UTP试剂在大鼠大脑中动脉(MCA)缺血30 min后以微量输注泵通过大鼠尾静脉持续给药,输注速度为5 ml/(kg.min)。再灌注24 h后测量大鼠脑含水量以及神经损伤行为学评分(NDS)。通过离体大鼠颈动脉环张力法测定UTP对去氧肾上腺素(PE)预收缩颈动脉环张力的影响。结果比较10、30、90μg/kg UTP组,发现UTP对大鼠脑缺血再灌注损伤的保护作用具有剂量依赖效应,90μg/kgUTP组的保护作用最明显。离体血管张力测定结果表明,UTP可以显著降低PE诱发的血管张力。结论UTP对大鼠脑缺血再灌注损伤具有保护作用,这种作用可能与梗塞部位的血管扩张有关。
Objective To observe the protective effect of uridine 5＇-triphosphate（UTP） on cerebral ischemia reperfusion（IR） injury in rats.Methods The injury model of middle cerebral artery occlusion（MCAO） was established by suture method.UTP were delivered through tail venousr via microinfusion pump 30 min after the occlusion of MCA at a rate of 5 ml/（kg·min）.Neurological deficit score（NDS） and brain water content were determined 24 h after reperfusion.The effect of UTP on the contraction of rat arteria carotis in vitro was examined.Results The protective effect of UTP on cerebral ischemia reperfusion showed a dose-dependent relationship.The 90 μg/kg group of UTP had the best protective effect among the 3 groups.The effect of UTP on the contraction of rat arteria carotis in vitro showed that UTP can significantly reduce the blood vessel tension induced by PE.Conclusion UTP has a dose-dependent protective effect on cerebral IR,which may be related to the expansion of the blood vessels of the infarct area.
出处
《药学实践杂志》
CAS
2010年第3期181-183,234,共4页
Journal of Pharmaceutical Practice
基金
上海市卫生局科研课题项目(2009125)
关键词
尿苷三磷酸
再灌注损伤
脑
uridine 5＇-triphosphate（UTP）
reperfusion injury
brain
