摘要
采用SRAP分子标记技术，对8份野生和8份栽培桔梗种质资源进行遗传多样性研究，旨在为桔梗的遗传育种提供理论依据。结果表明：12对随机引物组合共扩增出109奈谱带，平均每对引物组合扩增出9．1条谱带，其中扩增出80条多态性谱带，平均每对引物组合扩增出6．7条多态性谱带，多态率为73．4％，显示出了较高的多态性。试验中SRAP数据的遗传相似系数范围为0．56～0．88，并以遗传相似系数0．56为标准，将16份桔梗种质资源分为野生桔梗和栽培桔梗二大类群。
Sequence related amplified polymorphis（SRAP）molecular markers were used to detect the genetic diversity of 8 samples of the wild Playtcodon grandiflourus and 8 samples of the cultivation Playtcodon grandiflourus. The purpose of provide the basis for breedong of Playtcodon grandiflourus. The results showed that Twelve primer pairs produced 109 bands,veraged 9. 1 bands per primenr pair,and produced 80 polyrnorphic bands,veraged 6.7 polymorphic bands per primenr pair, showed a higher rate of polymorphic（73. 4%）. The SRAP data obtained genetic similarity coefficient range 0. 56~0. 88, the results showed that the 16 samples of Platycodon grandiflorum I3C. were clustered into the wild Playtcodon grandiflourus and the cultivation Playtcodon grandiflourus, two groups at genetic distance 0. 56.
出处
《北方园艺》
CAS
北大核心
2010年第12期132-135,共4页
Northern Horticulture
基金
国家自然科学基金资助项目(30660016)
关键词
桔梗
遗传多样性
SRAP标记
Playtcodon grandiflourus
genetic diversity
sequence related amplified polymorphis（SRAP）rnark
