摘要
目的:探讨解聚复肾宁(黄芪、地黄、黄连、丹参、姜黄、葛根等)(JJFSN)治疗早期糖尿病肾病(DN)的作用机制。方法:高糖诱导系膜细胞(MC)增殖以及细胞外基质(ECM)分泌,制备不同浓度JJFSN含药血清作用于体外培养的MC,用MTT法检测细胞增殖,放射免疫技术(RIA)检测细胞培养上清中Ⅳ型胶原(ColⅣ)及层黏连蛋白(Ln)的含量。结果:JJFSN含药血清可抑制高糖诱导的MC过度增殖(P<0.05),并且这种作用具有药物剂量和时间依赖关系。同时,JJFSN可逆转高糖对ColⅣ和Ln分泌的影响,降低MC培养上清中ColⅣ和Ln的含量(P<0.05),并且这种作用随着JJFSN剂量的增加而增强。结论:JJFSN抑制高糖诱导的MC过度增殖并减少ECM的过量分泌,可能是其防治DN早期的作用机制。
AIM： To investigate the effect of a traditional Chinese compound,Jiejufushening （Radix astragli;Radix rehmanniae;Rhizoma coptidis;Radix et Rhizoma salviae multiorrhizae;Rhizoma Curcumae longae;Radix puerariae lobatae,etc） （JJFSN） on proliferation and secretion of extracellular matrix（ECM） of rat mesangial cells （MC）.METHODS： Sera containing different dose of JJFSN were prepared and put together with 25mM glucose.MC was cultured in vitro for 12 h,24 h and 48 h respectively.The effects of the drug-containing sera on MC cell proliteration were assessed by the methylthiazoletetrazolium（MTT） assay.The supernatants were collected after 48 h growth.And collagen type Ⅳ （ Col Ⅳ） and laminin （ Ln） were detected by radioimmunoassay （ RIA）.RESULTS： JJFSN pharmacological serum significantly（P 0.05） inhibited MC proliferation under high glucose condition,in a dose and time-dependent manner.Meanwhile,compared with the high glucose group,the concentration of Col Ⅳ and Ln declined in different concentration JJFSN groups（P 0.05）,in a dose-dependent manner.CONCLUSION： JJFSN can supress the influence of high glucose on MC proliferation and secretion of Col Ⅳ and Ln.It implies that JJFSN might be a potential therapeutic agent for the prevention against DN in the early stage.
出处
《中成药》
CAS
CSCD
北大核心
2010年第6期918-921,共4页
Chinese Traditional Patent Medicine
基金
重庆市卫生局课题(2003-32-B-26)
重庆市教育委员会课题(2009-6-KJ090319)
关键词
解聚复肾宁
肾小球系膜细胞
糖尿病肾病
细胞外基质
Jiejufushenning （JJFSN）
diabetic nephropathy
mesangial cell
extracellular matrix
