摘要
目的:优选大孔吸附树脂纯化丹参酚酸的工艺条件。方法:以丹酚酸B的含量为指标,对大孔吸附树脂型号、吸附条件、洗脱条件进行了考察。结果:最佳工艺条件为采用HPD-300大孔吸附树脂,最大上样量以丹酚酸B计为172.40 mg.g-1干树脂,pH 3.5,以70%乙醇3倍柱体积洗脱,吸附-洗脱过程中丹酚酸B的平均保留率可达88.11%,纯化后丹酚酸B含量为72.80%。结论:该工艺可用于纯化富集丹酚酸类成分。
Objective：To study the optimum conditions for the purification of salvianolic acid by macro-porous resins.Method：The macroporous resin was selected and the purification process was evaluated by measuring the content of salvianolic acid B.Result：The HPD-300 maoroporous resin was the most effective one among the resins.The optimum conditions were screened,the adsorption capacity of HPD-300 macroporous resin was 172.4 mg/g,pH was 3.5;and the volume of 70% ethanol as eluent was 3 BV.By this method,the yield of salvianolic acid B was 88.11%;and the purity was 72.80%.Conclusion：The method is effective for purification of salvian-olic acid.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第6期14-16,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大专项(2009zx09103-407)
关键词
丹酚酸B
大孔吸附树脂
salvianolie acid B
macroporous resins
