摘要
目的:确定富集泽兰中酚酸类成分的大孔树脂的方法。方法:以原儿茶醛、咖啡酸和迷迭香酸为指标,在同一条件下比较10种不同树脂解吸附液中3个成分的HPLC色谱峰峰面积,优选树脂类型(HPD-600和AB-8河北);以不同洗脱部位固形物中这3个成分的含量为指标,确定乙醇洗脱剂浓度。结果:富集原儿茶醛,最佳树脂为HPD-600,洗脱溶剂为50%乙醇;富集咖啡酸,最佳树脂为HPD-600,洗脱溶剂为95%乙醇;富集迷迭香酸,最佳树脂为AB-8,洗脱溶剂为95%乙醇。结论:该试验为泽兰酚酸类成分富集工艺研究奠定基础,为泽兰的化学成分深入研究提供了关键技术。
Objective：The technology of enriching phenolic acids was researched from Lycopus lucidus Turcz by macroporods resins.Method：The peak areas of protocatechualdehyde,caffeic acid and rosmarinic acid were compared from the ten kinds of macroporous resins’desorbED solutions under the same HPLC condition to determine the kind of macroporous resins.The contents of protocatechualdehyde,caffeic acid and rosmarinic acid were chosen as assessment index to decide the concentration of ethanol.Result：The two kinds of macroporous resins,HPD-600 and AB-8 from Hebei province were optimized.The adsorption resin of HPD-600 was appropriate to enrich protocatechualdehyde and the eluant was 50% ethanol;the adsorption resin of HPD-600 was appropriate to enrich caffeic acid and the eluant was 95% ethanol;the adsorption resin of AB-8 was appropriate to enrich rosmarinic acid and the eluant was 95% ethanol.Conclusion：This experiencelaid a foundation for the enrichment technology of the phenolic acids from Herba Lycopi,it provided the key technology for the further research of Herba Lycopi’s consititutions.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第6期22-25,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
北京中医药大学科研基金项目(2008年度)
北京市教育委员会共建项目(2008年度)
关键词
大孔吸附树脂
泽兰
酚酸
macroporous resins
Herba Lycopi
phenolic acids
