摘要
目的观察针刺对脑挫伤模型大鼠脑组织坏死面积与脑细胞凋亡的影响。方法参照Feeney自由落体撞击造模法建立脑挫伤大鼠模型,将40只大鼠随机分为假手术组、模型组、纳络酮组、针刺组,每组10只。针刺组、纳络酮组分别给予针刺、腹腔注射纳络酮,每天1次,共7天。处理结束后,采用生物医学图像分析系统计算坏死面积、TUNEL法检测脑细胞凋亡。结果模型组大鼠脑组织损伤严重,坏死面积大于损伤面积,出现大量的脑细胞凋亡(P<0.01);针刺组、纳络酮组脑组织坏死面积较模型组明显减小,且均小于损伤面积(P<0.01);两组大鼠脑细胞凋亡指数较模型组明显减少(P<0.01);针刺组脑组织坏死面积与纳络酮组比较无统计学意义(P>0.05)。结论针刺可缩小脑挫伤模型大鼠脑织坏死面积、减少脑细胞凋亡,具有确切的抗继发性脑损伤作用。
Objective To observe the effect of acupuncture on the infracted area and apoptosis of rats with brain contusion. Methods Feeney＇s method was used to establish bl：ain contusion model. The 40 rats were randomized into sham-operation, model, acupuncture and naloxone （NX） group with 10 in each. Acupuncture was performed for the rats of acupuncture group and NX was in- jected intraperitoneally for the rats of NX group. The treatment was done once everyday, 7 days altogether. Then the brain histotomy was prepared after all the treatment finished. Computer image analysis system was used to detect the infracted area. TUNEL method was adopted to detect the apoptosis. Results The brain contusion in the model group was severe, there were lots of apoptotic cells and the infracted areas were bigger than the injured areas （P〈0.01）. The infracted areas of the acupuncture and NX groups were ob- viously smaller than those of the model group （P〈0.01） and smaller than the injured areas as well （P〈0. 01）; and the apoptotic cells of theses two treated groups were significantly less than those of the model group （P〈0.01）. There was no significant difference in the infracted areas between acupuncture and NX groups （P〉0. 05）. Conclusion Acupuncture can decrease the infracted areas and apoptosis of the rats with brain contusion, being effective in anti-secondary brain injuries.
出处
《中医杂志》
CSCD
北大核心
2010年第6期528-530,共3页
Journal of Traditional Chinese Medicine
基金
广东省自然科学基金资助项目(06300588)
广东省自然科学基金面上资助项目(9151063201000028)
暨南大学引进优秀人才科研启动基金资助项目(51207014)
关键词
脑挫伤
针灸疗法
坏死面积
细胞凋亡
大鼠
Brain contusion
Acupuncture therapy
Infracted area
Apoptosis
Rats
