摘要
目的利用AFLP技术分析香鳞毛蕨种质资源多样性。方法对46个香鳞毛蕨个体进行AFLP分析,利用NTSYS-PC 2.10、PopGene32软件分析各居群的遗传多样性。结果香鳞毛蕨居群多态位点百分率(PPB)为76.67%;观测等位基因数(Na)为1.766 7,有效等位基因数(Ne)为1.504 6,Ne′is基因多样性指数(H)为0.292 1,Shannon信息指数(I)为0.431 6;遗传分化指数(Gst)为0.412 5。结论香鳞毛蕨遗传多样性较高。群体内的基因多样性(Hs)为0.170 5,群体间的基因多样性(Dst)为0.119 8,且由于生境特殊性,对香鳞毛蕨资源应采取就地保护策略。
Objective To determine the genetic diversity of germplasm resource in Dryopteris fragrans by amplified fragment length polymorphism（AFLP） technique.Methods Forty-six samples from six populations of D.fragrans were analyzed by AFLP DNA markers,and the genetic diversity was evaluated by NTSYS-PC 2.10 and PopGen32.Results The percentage of polymorphic bands（PPB） reached to 76.67%,observed number of alleles（Na） was 1.766 7, effective number of alleles（Ne） was 1.504 6,Nei′s gene diversity index（H） was 0.292 1,Shannon information index（I） was 0.431 6,and genetic differentiation index（Gst） was 0.412 5.Conclusion Genetic diversity is high,gene diversity in populations（Hs） is 0.170 5,and gene diversity among populations（Dst） is 0.119 8.Because of environmental specificity,its resource on the spot should be protected.
出处
《中草药》
CAS
CSCD
北大核心
2010年第6期971-974,共4页
Chinese Traditional and Herbal Drugs
关键词
香鳞毛蕨
遗传多样性
AFLP
Dryopteris fragrans（L.） Schott
genetic diversity
amplified fragment length polymorphism（AFLP）
