摘要
目的 探讨血循环中Tie2 mRNA含量是否与脓毒症全身病情变化存在相关关系.方法 选取重症监护病房（ICU）创伤患者,按是否为脓毒症分为脓毒症组（13例）和非脓毒症组（19例）.患者进入ICU当日进行急性生理学与慢性健康状况评分系统Ⅱ（APACHEⅡ）评分;1、3、7 d采集肘静脉血检测白细胞总数（WBC）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、尿素氮（BUN）、肌酐（Cr）含量;采用实时定量聚合酶链反应（PCR）检测血中Tie2 mRNA含量;用酶联免疫吸附法检测血浆血管性血友病因子（vWF）含量.结果 脓毒症组与非脓毒症组间ALT、AST、vWF含量比较差异均无统计学意义[ALT（U/L）：53.30（199.58）比80.65（202.62）,AST（U/L）：316.53（49.90）比66.10（285.03）,vWF：（272.47±114.61）%比（246.66±128.77）%,均P＞0.05].脓毒症组WBC、BUN、Cr及血中Tie2 mRNA水平均显著高于非脓毒症组[WBC（×109/L）：18.26（21.82）比10.11（4.72）,BUN（mmol/L）：20.70（11.20）比7.70（5.45）,Cr（μmol/L）：252.00（364.55）比68.00（23.20）,Tie2 mRNA：1.86±0.67比0.91±0.42,均P＜0.01].所有患者APACHE Ⅱ评分与血Tie2 mRNA水平呈显著正相关（r=0.532,P＜0.01）,其线形拟合方程为Y=12.66＋4.922X（R2=0.283）vWF与Tie2 mRNA水平呈正相关（r=0.334,P＜0.05）,其线形拟合方程为Y=180.932＋57.93X（R2=0.112）.结论 脓毒症患者血循环中Tie2 mRNA水平能及时反映内皮细胞损伤程度和病情轻重,可作为一个相对敏感的病情预测候选指标.
Abstract：Objective To investigate the possibility that the level of Tie2 mRNA in peripheral blood could reflect the severity of sepsis.Methods Trauma patients in intensive care unit （ICU） were recruited,and they were divided into sepsis group （n=13） and non-sepsis group （n=19）.The severity of disease was evaluated with acute physiology and chronic health evaluation Ⅱ （APACHE Ⅱ） score on the day of ICU admission.Blood of patients was sampled on day 1, 3, 7 after ICU admission to determination of the white blood cell （WBC） count, contents of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）,blood urea nitrogen （BUN） and creatinine （Cr）, mRNA levels of Tie2 in blood measured by quantitative real-time polymerase chain reaction （PCR）, and the contents of plasma von Willebrand factor （vWF） with enzyme linked immunosorbent assay （ELISA）.Results No significant difference in contents of ALT, AST and plasma vWF was found between sepsis group and non-sepsis group [ALT （U/L）： 53.30 （199.58） vs.80.65 （202.62）, AST （U/L）： 316.53 （49.90）vs.66.10 （285.03）, vWF： （272.47 ±114.61）% vs.（246.66±128.77）%, all P〉0.05].The number of WBC [× 109/L, 18.26 （21.82） vs.10.11 （4.72）], the contents of BUN [mmol/L, 20.70 （11.20） vs.7.70 （5.45）] and Cr [μmol/L： 252.00 （364.55） vs.68.00（23.20）], and the circulating mRNA levels of Tie2 （1.86±0.67 vs.0.91±0.42） in sepsis group were higher than those in the non-sepsis group （all P〈0.01）.The Tie2 mRNA level in peripheral blood of each patient was positively correlated with APACHE I score （r= 0.532, P〈 0.01）.The linear regression equation was Y= 12.66＋ 4.922X （R2 = 0.283）.Besides, there was a significant correlation bettween the amount of Tie2 mRNA and plasma levels of vWF （r=0.334, P〈0.05）.The linear regression equation was Y=180.932＋57.93X （R2=0.112）.Conclusion The level of Tie2 mRNA in peripheral blood could reflect the damage of endothelial cell and severity of sepsis.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2010年第6期361-363,共3页
Chinese Critical Care Medicine
基金
基金项目：全军医药卫生科研基金项目（06G081）
关键词
TIE2
基因表达
实时定量聚合酶链反应
脓毒症
Tie2
Gene expression
Quantitative real-time polymerase chain reaction
Sepsis
