摘要
采用X射线荧光光谱(XRF)方法研究蛛丝和蚕丝的化学元素组成,探讨成丝机制中金属离子的作用及生物丝性能优良的元素基础。XRF分析蚕丝中C含量是47.10%、O为29.92%、N是16.52%;金属元素包括:Ca含量0.166 2%、Mg含量0.104 0%和K含量0.039 5%,而Na,Zn,Ni,Fe和Cr是微量元素。Ca和Mg元素含量高,在桑蚕吐丝机制中起着重要的作用。在蛛丝中,主要的非金属元素是:C含量44.09%、O含量26.64%和N含量22.34%。高含量的N元素可能是蛛丝优异的刚性和韧性性能的元素基础。在蛛丝中,Na含量0.268 0%、K含量0.081 4%和Mg含量0.011 6%,而Ca,Zn,Fe,Cu和Cr则是微量元素。蛛丝中Na和K元素含量高,在蜘蛛吐丝机制中可能起重要作用。运用数学统计方法研究生物丝元素组成与XRF元素分析结果吻合。
Elemental compositions in spider and silkworm silks were determined by X-ray fluorescence （XRF） spectrum to probe the silk-forming mechanisms and an elemental basis for spider silk with excellent characteristics. XRF analysis demonstrates that in the silkworm silk, the elemental content is 47. 10% for C, 29.92% for O and 16.52% for N, including metal elemental contents： 0. 166 2% for Ca, 0. 104 0% for Mg and 0. 039 5% for K, while Na, Zn, Ni, Fe and Cr show less micro quantity. Due to relative high quantity for Ca and Mg, they both play an important role in the silk-forming mechanism by silkworm. In the spider silk, the determined main nonmetal elemental contents are 44.09% for C, 26.64% for O and 22.34% for N. The high content of nitrogen may be an elemental basis for spider silk with excellent characteristic. The main metal elemental contents are 0. 268 0% for Na, 0. 081 4% for K and 0. 011 6% for Mg, while Ca, Zn, Ni, Cu and Cr possess less micro quantity in the spider silk. Because of relative high quantity for Na and K, they both play an important role in the silk-forming mechanism by spider. The elemental compositions investigated by using mathematic statistic method are quite in agreement with those demonstrated by using XRF spectrum, which validates the experimentally determined elemental compositions in the spider and silkworm silks.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2010年第7期1983-1989,共7页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(20272049)
中国科学院百人计划项目资助
关键词
蜘蛛
桑蚕
丝蛋白
XRF
元素组成
氨基酸
Spider
Silkworm
Silk protein
XRF
Elemental composition
Amino acid
