摘要
本文利用啤酒糟为主要原料,以杏鲍菇为菌种进行木聚糖酶发酵研究。通过单因素和正交试验确定最佳培养条件,并对其粗酶的酶学性质进行研究。结果表明,各因素对产酶能力的影响大小依次为:培养时间、料水比、氮源、pH、碳源。固态发酵的最佳培养条件为:碳源为以6∶2∶2配备的啤酒糟、玉米芯、麸皮混合物,氮源为1.5%的酵母膏,pH为5,装瓶量为7g/50ml三角瓶,接种量为25%,培养天数为9d,料水比为1∶1.6。其粗酶液的最适反应温度为50℃,最适pH为6。在pH为4～7时酶的稳定性较好,但木聚糖酶的热稳定性较差,60℃以上时酶活力损失70%以上。
In this paper brewer’s grains were used as main material to produce xylanase by Pleurotus eryngii. Monofactorial and orthogonal experiments were conducted to optimize the fermentation conditions,and the enzymatic characteristics of crude enzyme liquid were also studied. The results showed that the factors influencing xylanase yield from main to minor were incubation time,the ratio between material and water,nitrogen source,pH and carbon source. The optimal culture conditions for enzyme producing were： the mixture of brewer’s grains,corn cob and wheat bran of 6 ∶ 2∶ 2 as carbon source,1. 5% of yeast extract as nitrogen source,pH 5,7g medium in 50ml erlenmeyer flask,25% of inoculum’s volume for 9 days incubation,and the best ratio between material and water is 1∶ 1. 6. The optimal condition of the crude enzyme liquid is 50℃ ,pH6. The enzyme is stabile between pH 4 ～ 7,but its themal stability is poor,more than 70% of the enzyme activity lost above 60℃ .
出处
《核农学报》
CAS
CSCD
北大核心
2010年第3期542-547,共6页
Journal of Nuclear Agricultural Sciences
基金
安徽省教育厅自然科学基金(KJ2009B168Z)
安徽科技学院生物学重点建设学科项目
关键词
杏鲍菇
啤酒糟
固态发酵
木聚糖酶
正交试验
Pleurotus eyngii
brewer’s grain
solid state fermentation
xylanase
orthogonal experiment
