摘要
目的探讨噻唑蓝(MTT)法用于口腔常见细菌(变异链球菌、血链球菌、伴放线菌嗜血菌)活菌计数的可行性及具体应用过程中的实验条件。方法本实验以菌落形成单位(CFU)法为标准对照。实验通过改变比色的波长、反应时间、试剂剂量、细菌菌龄等实验条件,获得MTT法测量常见口腔细菌的一系列参数,对变异链球菌、血链球菌、伴放线菌嗜血菌进行活菌计数,并与CFU法所测的细菌数量相比较。结果 MTT法在测量变异链球菌时,最佳的检测波长为510nm,最佳的测定细菌范围是1.5×105～1.0×107CFU·mL-1。MTT法在测量血链球菌时,最佳的检测波长为545nm,最佳的测定细菌范围是1.5×105～2.0×107CFU·mL-1。MTT法在测量伴放线菌嗜血菌时,最佳的检测波长为557nm,最佳的测定细菌范围是1.0×106～5.0×107CFU·mL-1。MTT法与菌落形成单位法的测量结果是一致的,并且对于不同菌龄的变异链球菌、血链球菌、伴放线菌嗜血菌均适用。结论 MTT法可以用于检测变异链球菌、血链球菌、伴放线菌嗜血菌等口腔常见细菌的活菌数量,并且具有快速、方便等优点。
Objective To explore the feasibility of methyl thiazolyl tetrazolium（MTT） colorimetric method and the applied condition for the normal bacteria in the mouth, as Streptococcus mutans（S.mutans）, Streptococcus sanguis（S. sanguis）, Haemophilus actinomycetemcomitans（H.actinomycetemcomitans）. Methods Colony forming units（CFU） which was the standard antitheses was used to count bacteria. This study would gain some parameters by changing wavelength, reactive time, dosage and so on. MTT colorimetric method was applied in the counting of S.mutans, S. sanguis and H.actinomycetemcomitans. Results When counting S.mutans, the best wavelength was 510 nm, the best range was 1.5×10^5～1.0×10^7 CFU·mL^-1. When counting S.sanguis, the best wavelength was 545 nm, the best range was 1.5×10^5～2.0 ×10^7 CFU·mL ^-1. When counting H.actinomycetemcomitans, the best wavelength was 557 nm, the best range was 1.0×10^6～5.0×10^7 CFU·mL^-1. MTT colorimetric method can be used for different aged S.mutans, S.sanguis and H.actinomycetemcomitans. Conclusion Oral bacteria could be counted by MTT colorimetric method, which is fast and convenient.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2010年第3期306-310,共5页
West China Journal of Stomatology
关键词
噻唑蓝比色法
变异链球菌
血链球菌
伴放线菌嗜血菌
活菌计数
methyl thiazolyl tetrazolium colorimetric method
Streptococcus mutans
Streptococcus sanguis
Haemophilus actinomycetemcomitans
bacterial cell counting
